文献类型： 外文期刊

作者： Wang, Erlong ¹ ; Liu, Tao ¹ ; Wu, Jie ¹ ; Wang, Kaiyu ¹ ; Chen, Defang ³ ; Geng, Yi ¹ ; Huang, Xiaoli ³ ; Ouyang, Ping ¹ ; L ¹ ;

作者机构： 1.Sichuan Agr Univ, Coll Vet Med, Dept Basic Vet, Chengdu, Sichuan, Peoples R China

2.Sichuan Agr Univ, Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu, Sichuan, Peoples R China

3.Sichuan Agr Univ, Coll Anim Sci & Technol, Dept Aquaculture, Chengdu, Sichuan, Peoples R China

4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China

关键词： Interleukin-1 beta s; Molecular characterization; Adjuvant effect; pSCPI; Channel catfish

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2019 年 87 卷

页码：

收录情况： SCI

摘要： Channel catfish is one of the most extensively cultured species worldwide, which is widely used as a classical model for comparative immunology. Interleukin-1 beta(IL1 beta) is an immunoregulatory cytokine with the potential to enhance the immune response induced by vaccines in many animals. To characterize the molecular characterization and identify the immunoadjuvant role of channel catfish IL1 beta, molecular cloning, phylogenetic analysis, and expression of two IL1 beta genes were performed, the bioactivity of their recombinant proteins (rIL1 beta 1 and rIL1 beta 2) were detected in vitro and their adjuvant effects on a subunit vaccine encoding C5a peptidase (pSCPI) of Streptococcus iniae were evaluated. The results indicated that two IL1 beta s remained highly conserved possessing five conserved motifs compared with other fish IL1 beta s, although there were 28 nucleotide differences and 16 amino acid differences between channel catfish IL1 beta 1 and IL1 beta 2. Analysis of the ratios of nonsynonymous (dN) and synonymous (dS) substitutions revealed that fish IL1 beta genes were subjected to negative/purifying selection with global dN/dS ratios value 0.425. The results of adjuvant effect showed that compared with injection of pSCPI alone, co-injecting pSCPI with both HUN and rIL1 beta 2 significantly enhanced antibody levels, serum bactericidal activity, lysozyme activity, alternative complement hemolytic activity, and the expression of endogenous IL1 beta and TNF-alpha in head kidney and spleen. Although vaccination with rIL1 beta 1 or rIL1 beta 2 failed to offer immunoprotection against S. iniae infection, the RPS (relative percent survival) of pSCPI+ rIL1 beta 1 and pSCPI + rIL1 beta 2 groups were both higher than pSCPI alone (BPS, 50%), with 64.26% and 60.71%, respectively. Moreover, pSCPI+ rIL1 beta 1+ rIL1 beta 2 offered significantly higher (P < 0.05) immunoprotection (RPS, 75%) against S. iniae infection than pSCPI alone. Our present results not only enrich the molecular structure study of fish IL1 beta s but also signify that two recombinant channel catfish IL1 beta s can be used as potential adjuvants in a subunit vaccine model against bacterial infection, which are of profound importance to prevent and control bacterial disease in channel catfish.