pik3r3b, a novel immune-related gene in Nile tilapia (Oreochromis niloticus): Identification, expression and analysis of antibacterial activity
文献类型： 外文期刊
作者： Meng, Liang 1 ; Xu, Wen-teng 1 ; Chen, Ya-dong 1 ; Wei, Min 1 ; Cui, Zhong-kai 1 ; Liu, Yang 1 ; Guo, Hua 1 ; Gan, Xi 4 ; Zh 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
3.Shandong Key Lab Marine Fishery Biotechnol & Gene, Qingdao 266071, Shandong, Peoples R China
4.Guangxi Acad Fisheries Sci, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Nanning 530021, Peoples R China
关键词： Phosphatidylinositol 3-kinase regulatory subunit gamma; Nile tilapia; Expression pattern; Immune response; Streptococcus agalactiae
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2019 年 87 卷
页码：
收录情况： SCI
摘要： A full-length cDNA encoding phosphatidylinositol 3-kinase regulatory subunit gamma b gene in Nile tilapia (Oreochromis niloticus), termed as On-pik3r3b, was identified and characterized in this study. The sequence analysis demonstrated that the full-length cDNA of On-pik3r3b was 2018 bp, containing a 5' untranslated region (UTR) of 171 bp, an open reading frame (ORF) of 1422 bp and a 3' UTR of 425 bp. Its protein sequence displayed a high degree of identity with other fish. Using qPCR, the expression patterns of On-pik3r3b were investigated. In healthy Nile tilapia, the transcripts of On-pik3r3b were detected in all examined tissues, except the skin. Upon the stimulation with Streptococcus agalactiae, the On pik3r3b expression level in liver, spleen, kidney and gill were significantly increased at 12 h after infection. The recombinant On-pik3r3b showed in vitro antibacterial activity, against S. agalactiae and E. coli. Our observation strongly indicates that On-pik3r3b is involved in the innate immune response in Nile tilapia.
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