Development of an enzyme-linked immunosorbent assay and a dipstick assay for the rapid analysis of trans-resveratrol in grape berries
文献类型： 外文期刊
作者： Chen, Xiaojiao 1 ; He, Junhu 3 ; Tan, Guiyu 1 ; Liang, Ju 4 ; Hou, Yuxia 2 ; Wang, Mian 1 ; Wang, Baomin 1 ;
作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China
2.China Agr Univ, Coll Sci, Beijing 100193, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Hainan, Peoples R China
4.Xinjiang Acad Agr Sci, Tulufan Inst Agr Sci, Tulufan 838000, Xinjiang, Peoples R China
关键词： trans-Resveratrol; Enzyme-linked immunosorbent assay; Lateral flow immunoassay; Grape berries; Polyphenol
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2019 年 291 卷
页码：
收录情况： SCI
摘要： trans-Resveratrol, a natural polyphenol present in grapes, has many beneficial effects to human health. However, measurement of trans-resveratrol is technically complicated and time-consuming. In the present study, we obtained a sensitive and specific monoclonal antibody (mAb), namely 3C9, against trans-resveratrol. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed, with 50% inhibitory concentration and working range of 1.0 ng/mL and 0.19-4.9 ng/mL, respectively. A lateral flow immunoassay (LFIA) based on 3C9 was also developed for the semiquantitative detection of trans-resveratrol in an indicator range of 50-100 ng/mL. Average recoveries of trans-resveratrol spiked in red and green grape berries samples were respectively 88-107% and 83-102% by icELISA. The icELISA and LFIA were applied for determination of transresveratrol in grape berries. The results were highly consistent with those determined by high-performance liquid chromatography analysis (R-2 = 0.9997). Therefore, we conclude that the immunoassay methods are suitable for the large-scale screening of trans-resveratrol in grape quality breeding.
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