Selective enrichment and quantification of okadaic acid in shellfish using an immunomagnetic-bead-based liquid chromatography with tandem mass spectrometry assay
文献类型： 外文期刊
作者： Chen, Jiaqi 1 ; Tan, Zhijun 1 ; Wu, Haiyan 1 ; Peng, Jixing 1 ; Zhai, Yuxiu 1 ; Guo, Mengmeng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Qingdao, Shandong, Peoples R China
2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Shandong, Peoples R China
关键词： immunoaffinity; liquid chromatography; okadaic acid; protein G magnetic beads; tandem mass spectrometry
期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）
ISSN： 1615-9306
年卷期： 2019 年 42 卷 7 期
页码：
收录情况： SCI
摘要： Okadaic acid is a marine biotoxin that primarily occurs in shellfish and can cause diarrheic shellfish poisoning in humans. When analyzing biological samples using liquid chromatography with tandem mass spectrometry, the presence of complex matrices is a major issue. Thus, it is crucial to selectively and simply extract the target analyte from samples and minimize matrix effects simultaneously. To meet this need, an immunomagnetic- bead- based liquid chromatography with tandem mass spectrometry method was developed to detect okadaic acid in shellfish. Magnetic beads bound to monoclonal antibody against okadaic acid were used as affinity probes to specifically enrich okadaic acid in samples, which effectively eliminated matrix effects. A magnetic separator was used to aggregate and separate magnetic particles from sample matrices, and methanol was used to elute okadaic acid from the magnetic beads. Standard solution prepared with methanolwas employed directly for quantitative analysis. Several experimental conditions were optimized to improve performance. The method is of interest as a rapid (10 min) sample clean-up and selective enrichment tool, and it showed good linearity and sensitivity, with reported limits of detection and quantitation of 3 and 10 mu g/kg, respectively. Fifty-three shellfish samples from an aquatic products market were tested using this method, and four samples positive for okadaic acid were found.
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