The stress hormone norepinephrine increases the growth and virulence of Aeromonas hydrophila
文献类型： 外文期刊
作者： Gao, Jinwei 1 ; Xi, Bingwen 1 ; Chen, Kai 1 ; Song, Rui 2 ; Qin, Ting 1 ; Xie, Jun 1 ; Pan, Liangkun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi, Jiangsu, Peoples R China
2.Hunan Fisheries Sci Inst, Changsha, Hunan, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
关键词： Aeromonas hydrophila; growth; norepinephrine; stress; virulence
期刊名称：MICROBIOLOGYOPEN （ 影响因子：3.139； 五年影响因子：3.503 ）
ISSN： 2045-8827
年卷期： 2019 年 8 卷 4 期
页码：
收录情况： SCI
摘要： Stress is an important contributing factor in the outbreak of infectious fish diseases. To comprehensively understand the impact of catecholamine stress hormone norepinephrine (NE) on the pathogenicity of Aeromonas hydrophila, we assessed variations in bacterial growth, virulence-related genes expression and virulence factors activity after NE addition in serum-SAPI medium. Further, we assessed the effects of NE on A. hydrophila virulence in vivo by challenging fish with pathogenic strain AH196 and following with or without NE injection. The NE-associated stimulation of A. hydrophila strain growth was not linear-dose-dependent, and only 100 mu M, or higher concentrations, could stimulate growth. Real-time PCR analyses revealed that NE notably changed 13 out of the 16 virulence-associated genes (e.g. ompW, ahp, aha, ela, ahyR, ompA, and fur) expression, which were all significantly upregulated in A. hydrophila AH196 (p < 0.01). NE could enhance the protease activity, but not affect the lipase activity, hemolysis, and motility. Further, the mortality of crucian carp challenged with A. hydrophila AH196 was significantly higher in the group treated with NE (p < 0.01). Collectively, our results showed that NE enhanced the growth and virulence of pathogenic bacterium A. hydrophila.
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