您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Nonadditive and allele-specific expression of insulin-like growth factor 1 in Nile tilapia (Oreochromis niloticus, female) x blue tilapia (O. aureus, male) hybrids

文献类型： 外文期刊

作者： Zhou, Yi 1 ; Zhang, Xiaojin 1 ; Xu, Qian 3 ; Yan, Jinpeng 3 ; Yu, Fan 4 ; Wang, Fenghua 5 ; Xiao, Jun 1 ; Luo, Yongju 1 ; Zh 1 ;

作者机构： 1.Guangxi Acad Fishery Sci, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Nanning 530021, Guangxi, Peoples R China

2.Shanghai Ocean Univ, Key Lab Aquat Genet Resources & Utilizat, Minist Agr, Shanghai 201306, Peoples R China

3.Cent S Univ, Sch Life Sci, Dept Cell Biol, Changsha 410017, Hunan, Peoples R China

4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Lab Freshwater Fisheries & Germplasm Resources Ut, Minist Agr, Wuxi 214081, Peoples R China

5.Xinjiang Normal Univ, Inst Phys Educ, Sports Biochem Lab, Urumqi 830054, Peoples R China

关键词： Tilapia; Heterosis; Allele-specific expression; Pyrosequencing; Nonadditive expression; IGF-1

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2019 年 232 卷

页码：

收录情况： SCI

摘要： Hybrid Nile tilapia (Oreochromis niloticus, female) x blue tilapia (O. aureus, male) is a widely cultured tilapia variety due to its growth vigor compared to the parent species. As a peptide hormone, insulin-like growth factor 1 (IGF-1) plays a critical role in regulating somatic growth. The present study focuses on the expression characteristics of IGF-1 in hybrid tilapia. The cloned complete open reading frame of IGF-1 in hybrid tilapia is 549 bp in length, encoding a protein of 182 amino acids. The deduced protein is highly similar to that of Nile tilapia and blue tilapia. IGF-1 was found to be primarily expressed in the liver and muscle in the hybrid; lower expression levels were found in other tissues such as the intestine, spleen, and head-kidney. Increased mRNA expression was observed in the liver and muscle of the hybrid compared to Nile tilapia and blue tilapia, indicating a nonadditive expression pattern in the hybrid. An IGF-1 SNP site (397 site: C in Nile tilapia, G in blue tilapia) for differentiating the Nile tilapia or blue tilapia subgenome in hybrids was identified. Pyrosequencing analysis of the liver transcriptome indicated that most of the hybrids (9 of 10 individuals) predominantly expressed the G allele, demonstrating bias of the blue tilapia subgenome. The present study provides novel data indicating, for the first time, overall gene expression of IGF-1 and allele-specific expression in hybrid tilapia.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台