Molecular cloning, expression pattern analysis, and in situ hybridization of a Transformer-2 gene in the oriental freshwater prawn, Macrobrachium nipponense (de Haan, 1849)
文献类型： 外文期刊
作者： Wang, Yabing 1 ; Jin, Shubo 2 ; Fu, Hongtuo 1 ; Qiao, Hui 2 ; Sun, Shengming 2 ; Zhang, Wenyi 2 ; Jiang, Sufei 2 ; Gong, 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China
关键词： Macrobrachium nipponense; Transformer-2; Temporal and spatial expression; In situ hybridization
期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）
ISSN： 2190-572X
年卷期： 2019 年 9 卷 6 期
页码：
收录情况： SCI
摘要： In this study, we isolated a full-length cDNA sequence from Macrobrachium nipponense and investigated its gene function. We named the gene Mntra-2a because of high similarities and close evolutionary divergence with arthropod tra-2. The full-length cDNA of Mntra-2a was 1293bp, consisting of a 212bp 5 UTR, a 268bp 3 UTR, and an ORF of 813bp encoding 270 amino acids. It contained an RNA recognition motif and a linker region. Real-time PCR analysis showed that Mntra-2a was highly expressed in the gonads of both males and females. Further in situ hybridization analysis showed that Mntra-2a was mainly located in oocytes and spermatocytes. During embryogenesis, Mntra-2a expression was higher in the cleavage and nauplius stages. During the ovarian reproductive cycle, Mntra-2a expression reached a peak at OvaryV and decreased to the lowest level at OvaryIV. These results indicated that Mntra-2a probably played important roles in embryonic development and early gonad development in M. nipponense. Our results provide basic information for further functional studies of tra-2 in M. nipponense.
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