文献类型： 外文期刊
作者： Yang, Ruopeng 1 ; Shang, Xiyu 2 ; Wang, Chuanming 1 ; Li, Mijie 1 ; Jiang, Chengyan 1 ; Lu, Haiju 1 ; Kong, Qiong 1 ; Gao 1 ;
作者机构： 1.Honghe Univ, Yunnan Higher Educ Inst, Key Lab Crops High Qual & Efficient Cultivat & Se, Mengzi 661400, Peoples R China
2.NanYang Med Coll, Dept Basic Med, Nanyang, Peoples R China
3.South China Agr Univ, Coll Anim Sci, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
关键词： Autophagy; Sf9; Spodoptera frugiperda; starvation
期刊名称：PHYSIOLOGICAL ENTOMOLOGY （ 影响因子：1.833； 五年影响因子：1.72 ）
ISSN： 0307-6962
年卷期： 2019 年 44 卷 2 期
页码：
收录情况： SCI
摘要： Autophagy controls insect development and can be targeted for pest control in agriculture. In the present study, starvation-induced autophagy is investigated in the insect species Spodoptera frugiperda. Bioinformatics analysis and a search of the EST database (http://bioweb.ensam.inra.fr/spodobase) identifies a putative ATG8 gene of S. frugiperda. To generate a biomarker of autophagosome, the DNA sequence encoding the open reading frame of this gene is amplified and cloned into a pIEX-4-mCherry-EGFP-SfATG8 recombinant vector. Sf9 cells are then transfected with this expression vector and starved in phosphate-buffered saline solution for 4 h to induce autophagy, which is examined by LysoTracker staining (Life Technologies, Grand Island, New York), western blotting and fluorescence microscopy. The results obtained show that starvation stimulates lipidation of SfATG8-PE and the formation of autophagosomes, providing a foundation for further research with respect to autophagy in insects.
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