文献类型： 外文期刊
作者： Yang, Ruopeng 1 ; Shang, Xiyu 2 ; Wang, Chuanming 1 ; Li, Mijie 1 ; Jiang, Chengyan 1 ; Lu, Haiju 1 ; Kong, Qiong 1 ; Gao 1 ;
作者机构： 1.Honghe Univ, Yunnan Higher Educ Inst, Key Lab Crops High Qual & Efficient Cultivat & Se, Mengzi 661400, Peoples R China
2.NanYang Med Coll, Dept Basic Med, Nanyang, Peoples R China
3.South China Agr Univ, Coll Anim Sci, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
关键词： Autophagy; Sf9; Spodoptera frugiperda; starvation
期刊名称：PHYSIOLOGICAL ENTOMOLOGY （ 影响因子：1.833； 五年影响因子：1.72 ）
ISSN： 0307-6962
年卷期： 2019 年 44 卷 2 期
页码：
收录情况： SCI
摘要： Autophagy controls insect development and can be targeted for pest control in agriculture. In the present study, starvation-induced autophagy is investigated in the insect species Spodoptera frugiperda. Bioinformatics analysis and a search of the EST database (http://bioweb.ensam.inra.fr/spodobase) identifies a putative ATG8 gene of S. frugiperda. To generate a biomarker of autophagosome, the DNA sequence encoding the open reading frame of this gene is amplified and cloned into a pIEX-4-mCherry-EGFP-SfATG8 recombinant vector. Sf9 cells are then transfected with this expression vector and starved in phosphate-buffered saline solution for 4 h to induce autophagy, which is examined by LysoTracker staining (Life Technologies, Grand Island, New York), western blotting and fluorescence microscopy. The results obtained show that starvation stimulates lipidation of SfATG8-PE and the formation of autophagosomes, providing a foundation for further research with respect to autophagy in insects.
- 相关文献
作者其他论文 更多>>
-
Novel perspectives on autophagy-oxidative stress-inflammation axis in the orchestration of adipogenesis
作者：Hong, Chun;Li, Xinming;Huang, Qiuyan;Li, Baohong;Xin, Haiyun;Hu, Bin;Meng, Fanming;Li, Jianhao;Wang, Sutian;Zhang, Kunli;Huang, Qiuyan;Hu, Chuanhuo;Zhu, Xiangxing;Tang, Dongsheng;Hu, Chuanhuo;Tao, Chenyu;Cao, Yang;Wang, Hai;Deng, Bo;Wang, Sutian
关键词：adipogenesis; autophagy; inflammation; oxidative stress; immune responses
-
Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
作者：Cao, Yang;Yu, Yongsheng;Zhang, Lichun;Liu, Yu;Jin, Haiguo;Liu, Lixiang;Cao, Yang;Zheng, Kaizhi;Wang, Sutian
关键词：ACSL1; transcript variants; sheep; APA; lipid metabolism
-
Transcriptome analysis reveals potential function of long non-coding RNAs in 20-hydroxyecdysone regulated autophagy in Bombyx mori
作者：Qiao, Huili;Wang, Jingya;Wang, Yuanzhuo;Yang, Juanjuan;Wei, Bofan;Li, Miaomiao;Wang, Bo;Li, Xiaozhe;Li, Dandan;Yao, Lunguang;Kan, Yunchao;Wang, Jingya;Li, Miaomiao;Cao, Yang;Cao, Yang;Tian, Ling
关键词：LncRNA; Transcriptome; 20-hydroxyecdysone; Autophagy-related gene; Silkworm
-
Toll9 from Bombyx mori functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals
作者：Zhang, Ruonan;Li, Xiaofeng;Wang, Yuan;Song, Yuhang;Ren, Feifei;Yi, Huiyu;Deng, Xiaojuan;Zhong, Yangjin;Cao, Yang;Yang, Wanying;Zhang, Ruonan;Zhang, Jie;Li, Yanjun;Yu, Xiao-Qiang;Cao, Yang;Cao, Yang;Strand, Michael R.
关键词：immunity; lipopolysaccharide; ML proteins; MD-2; Toll
-
P300/HDAC1 regulates the acetylation/deacetylation and autophagic activities of LC3/Atg8-PE ubiquitin-like system
作者：Wu, Wenmei;Guo, Sanyou;Xu, Jing;Ma, Qiuqin;Li, Shuyan;Xu, Xianying;Huang, Zhijun;Zhong, Yangjin;Tian, Ling;Wu, Wenmei;Guo, Sanyou;Xu, Jing;Ma, Qiuqin;Li, Shuyan;Xu, Xianying;Huang, Zhijun;Zhong, Yangjin;Tian, Ling;Li, Kang;Li, Sheng;Tettamanti, Gianluca;Tettamanti, Gianluca;Cao, Yang;Cao, Yang
关键词：
-
Steroid hormone 20-hydroxyecdysone induces the transcription and complex assembly of V-ATPases to facilitate autophagy in Bombyx mori
作者：Dai, Yichen;Wu, Wenmei;Wu, Kunzhong;Yi, Huiyu;Li, Wangyu;Zhong, Yangjin;Cao, Yang;Tian, Ling;Li, Kang;Xiao, Yang;Cao, Yang
关键词：20-Hydroxyecdysone; Starvation; BmV-ATPases; BmTFEB; BmmTORCI; Autophagic flux
-
Effects of Long-Chain Fatty Acyl-CoA Synthetase 1 on Diglyceride Synthesis and Arachidonic Acid Metabolism in Sheep Adipocytes
作者：Cao, Yang;Liu, Shunqi;Lian, Zhengxing;Cao, Yang;Wang, Yanli;Jin, Haiguo;Ma, Huihai;Luo, Xiaotong;Cao, Yang;Wang, Sutian
关键词：ACSL1; RNA-seq; lipid metabolome; arachidonic acid; COX2
