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High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions

文献类型： 外文期刊

作者： Yu, Xin-Jun 1 ; Huang, Chang-Yi 1 ; Chen, Hong 1 ; Wang, Dong-Sheng 2 ; Chen, Jing-Liang 1 ; Li, Hui-Juan 3 ; Liu, Xia 1 ;

作者机构： 1.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Key Lab Bioorgan Synth Zhejiang Prov, 18 Chaowang Rd, Hangzhou 310014, Zhejiang, Peoples R China

2.Jiangxi Acad Sci, Inst Biol Resources, Nanchang 330096, Jiangxi, Peoples R China

3.Shandong Univ Sci & Technol, Dept Bioengn, Coll Chem & Environm Engn, Qingdao 266590, Shandong, Peoples R China

4.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian 223300, Jiangsu, Peoples R China

5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China

关键词： Aurantiochytrium sp; carbohydrate; DHA; feed; FT-IR; high-throughput

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2019 年 24 卷 8 期

页码：

收录情况： SCI

摘要： The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protist Aurantiochytrium sp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109 Aurantiochytrium sp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the two Aurantiochytrium sp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginous Aurantiochytrium sp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.

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