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Transcriptomic Mechanism of the Phytohormone 6-Benzylaminopurine (6-BAP) Stimulating Lipid and DHA Synthesis in Aurantiochytrium sp.

文献类型： 外文期刊

作者： Yu, Xin-Jun 1 ; Chen, Hong 1 ; Huang, Chang-Yi 1 ; Zhu, Xiao-Yu 1 ; Wang, Zhi-Peng 2 ; Wang, Dong-Sheng 3 ; Liu, Xiao- 1 ;

作者机构： 1.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Key Lab Bioorgan Synth Zhejiang Prov, 18 Chaowang Rd, Hangzhou 310014, Zhejiang, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China

3.Jiangxi Acad Sci, Inst Biol Resources, Nanchang 330096, Jiangxi, Peoples R China

4.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian 223300, Peoples R China

5.Shandong Univ Sci & Technol, Coll Chem & Environm Engn, Dept Bioengn, Qingdao 266590, Shandong, Peoples R China

关键词： Aurantiochytrium sp; 6-benzylaminopurine; RNA-seq; DHA; transcription factor

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 19 期

页码：

收录情况： SCI

摘要： The phytohormone 6-benzylaminopurine (6-BAP) significantly improves lipid synthesis of oleaginous microorganisms with the great potential applied in lipid production. In the current study, the lipid and DHA productions in oleaginous Aurantiochytrium sp. were found to be improved by 48.7% and 55.3%, respectively, induced by 6-BAP treatments. Then, using high-throughput RNA-seq technology, the overall de novo assembly of the cDNA sequence data generated 53871 unigenes, and 15902 of these were annotated in at least one database. The comparative transcriptomic profiles of cells with and without 6-BAP treatments revealed that a total of 717 were differently expressed genes (DE), with 472 upregulated and 245 downregulated. Further annotation and categorization indicated that some DE genes were involved in pathways crucial to lipid and DHA productions, such as fatty acid synthesis, central carbon metabolism, transcriptional factor, signal transduction, and mevalonate pathway. A regulation mode of 6-BAP, in turn, perception and transduction of 6-BAP signal, transcription factor, expression regulations of the downstream genes, and metabolic changes, respectively, was put forward for the first time in the present study. This research illuminates the transcriptomic mechanism of phytohormone stimulation of lipid and DHA production in an oleaginous microorganism and provides the potential targets modified using genetic engineering for improving lipid and DHA productivity.

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