文献类型： 外文期刊
作者： Wu, Xiaowen 1 ; Zhang, Yingqing 1 ; Xu, Shumei 1 ; Chang, Yuyang 1 ; Ye, Yang 3 ; Guo, Anning 1 ; Kang, Yi 1 ; Guo, Haiya 1 ;
作者机构： 1.Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China
2.Shanghai Ocean Univ, Shanghai Collaborat Innovat Aquat Anim Genet & Br, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Obstet & Gynecol, Yanjiang Rd 107, Guangzhou 510120, Guangdong, Peoples R China
4.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 51380, Guangdong, Peoples R China
关键词： Gonadal soma-derived factor (Gsdf); Insulin-like growth factor 2 mRNA binding protein 3 (Igf2bp3); Trimethylated histone H3 lysine 27 (H3K27me3)
期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）
ISSN： 0016-6480
年卷期： 2019 年 277 卷
页码：
收录情况： SCI
摘要： Gonadal soma-derived factor (Gsdf) is a unique TGF-f3 factor essential for both ovarian and testicular development in Hd-rR medaka (Oryzias latipes). However, the downstream genes regulated by Gsdf signaling remain unknown. Using a high-throughput proteomic approach, we identified a significant increase in the expression of the RNA-binding protein Igf2bp3 in gsdf-deficient ovaries. We verified this difference in transcription and protein expression against normal gonads using real-time PCR quantification and Western blotting. The genomic structure of igf2bp3 and the syntenic flanking segments are highly conserved across fish and mammals. igf2bp3 expression was correlated with oocyte development, which is consistent with the expression of the igf2bp3 ortholog Vg1-RBP/Vera in Xenopus. In contrast to the normal ovary, cysts of H3K27me3- and Igf2bp3-positive germ cells were dramatically increased in the one-month-old gsdf-deficient ovary, indicating that the gsdf depletion led to a dysregulation of Igf2bp3-mediated oocyte development. Our results provide novel insights into the Gsdf-Igf2bp3 signaling mechanisms that underlie the fundamental process of gametogenesis; these mechanisms may be well conserved across phyla.
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