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Cyclic peptide: a safe and effective alternative to synthetic aflatoxin B-1-competitive antigens

文献类型： 外文期刊

作者： Mukhtar, Hina 1 ; Ma, Lan 1 ; Pang, Qian 1 ; Zhou, Youxiang 2 ; Wang, Xiaohong 1 ; Xu, Ting 3 ; Hammock, Bruce D. 4 ; Wan 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Wuhan 430064, Hubei, Peoples R China

3.China Agr Univ, Coll Resources & Environm Sci, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

4.Univ Calif Davis, Dept Entomol, 96 Briggs Hall, Davis, CA 95616 USA

5.Univ Calif Davis, UCD Comprehens Canc Ctr, 96 Briggs Hall, Davis, CA 95616 USA

关键词： Aflatoxin B-1; Phage display; Cyclic peptide; Immunoassay

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2019 年 411 卷 17 期

页码：

收录情况： SCI

摘要： Aflatoxin B-1 (AFB(1)) is one of the major mycotoxins, which naturally occurs in food and agricultural products. In this study, a cyclic peptide (CVPSKPGLC) mimicking AFB(1) was used to develop a biotinylated peptide-based immunoassay (bp-ELISA) for AFB(1) determination. This cyclic peptide was isolated from a commercially available phage-displayed random 7-mer cyclic peptide library, and then synthesized chemically. Instead of phage particles, the peptide was biotinylated and used to detect AFB(1) by bp-ELISA, with an IC50 of 0.92ng/mL, which was approximately 60-fold better than that of phage ELISA. Good recoveries (83-102%) were obtained in spiked rice and corn samples, which were further validated by high-performance liquid chromatography-fluorescence detector. As better sensitivities (0.92-1.21ng/mL) were obtained by bp-ELISA even using selected anti-AFB(1) antibodies prepared previously in laboratory, this cyclic peptide is suitable as a substitute for synthetic competitive AFB(1) antigens in food contamination monitoring.

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