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Fine mapping and candidate gene analysis of the yellow petal gene ckpc in Chinese kale (Brassica oleracea L. var. alboglabra Bailey) by whole-genome resequencing

文献类型： 外文期刊

作者： Xu, XiaoMei 1 ; Luo, Wenlong 1 ; Guo, Juxian 1 ; Chen, Hancai 1 ; Akram, Waheed 1 ; Xie, Dasen 1 ; Li, Guihua 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China

关键词： Chinese kale; Fine mapping; Candidate gene; Petal color; Whole-genome resequencing

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2019 年 39 卷 7 期

页码：

收录情况： SCI

摘要： Chinese kale is a characteristic vegetable crop originating from China and being suggested to be a possible ancestor of various cultivated B. oleracea vegetables. It has both white and yellow petals and is therefore a desirable plant for use in research on petal color formation. In our study, the Chinese kale landrace YCK-1 (yellow petals) was crossed with the Chinese kale inbred line Lb07M (white petals) to construct mapping populations for genetic analysis and fine mapping of the petal color gene. The results indicated that yellow petal color is controlled by a single recessive gene named ckpc, which was preliminarily located in a 2.06-Mb candidate region of chromosome C03 using whole-genome resequencing combined with bulked segregant analysis (BSA). Fine mapping with traditional molecular marker technology was used to narrow down the ckpc gene in a 69.0-kb interval flanked by the markers M916 and SNP4 at genetic distances of 0.07cM and 0.04cM, respectively. Gene annotation revealed 5 genes in that region, and Bo3g158650 was annotated as a carotenoid cleavage dioxygenase 4 (CCD4) gene, which is known to function in flower and flesh color formation. Sequence analysis revealed that the Bo3g158650 gene in the white-petaled parent, Lb07M, had an identical sequence to that in the TO1000' reference genome, while a 7.61-kb CACTA-like transposable element 1 (TE1) insertion was found in this gene in the yellow-petaled parent, YCK-1. These results increase the understanding of the mechanism underlying petal color formation in Brassica.

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