文献类型： 外文期刊

作者： Barkham, Timothy ¹ ; Zadoks, Ruth N. ² ; Amal Azmai, Mohammad Noor ³ ; Baker, Stephen ⁵ ; Vu Thi Ngoc Bich ⁶ ; Chal ¹ ;

作者机构： 1.Tan Tock Seng Hosp, Dept Lab Med, Singapore, Singapore

2.Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Coll Med Vet & Life Sci, Glasgow, Lanark, Scotland

3.Univ Putra Malaysia, Dept Biol, Fac Sci, Serdang, Malaysia

4.Univ Putra Malaysia, Lab Marine Biotechnol, Inst Biosci, Serdang, Malaysia

5.Univ Oxford, Clin Res Unit, Ho Chi Minh City, Vietnam

6.Univ Oxford, Clin Res Unit, Ctr Trop Med, Hanoi, Vietnam

7.Publ Hlth England, London, England

8.Natl Environm Agcy, Environm Hlth Inst, Singapore, Singapore

9.Singapore Food Agcy, Natl Ctr Food Sci, Singapore, Singapore

10.Mahosot Hosp, Lao Oxford Mahosot Hosp Wellcome Trust Res Unit, Viangchan, Laos

11.Univ Oxford, Nuffield Dept Med, Ctr Trop Med & Global Hlth, Oxford, England

12.London Sch Hyg & Trop Med, Fac Infect & Trop Dis, London, England

13.Khoo Teck Puat Hosp, Dept Lab Med, Singapore, Singapore

14.Univ Oxford, Clin Res Unit, Hanoi, Vietnam

15.Univ Oxford, Nuffield Dept Clin Med, Ctr Trop Med & Global Hlth, Oxford, England

16.Natl Ctr Infect Dis, Singapore, Singapore

17.Univ Cambridge, Dept Vet Med, Cambridge, England

18.Singapore Gen Hosp, Dept Microbiol, Singapore, Singapore

19.Univ Lisbon, Inst Microbiol, Inst Med Mol, Fac Med, Lisbon, Portugal

20.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore, Singapore

21.Hue Univ, Univ Agr & Forestry, Fac Fisheries, Hue City, Vietnam

22.Thailand Minist Publ Hlth MOPH US Ctr Dis Control, Nonthaburi, Thailand

23.Nakhon Phanom Gen Hosp, Nakhon Phanom Prov Hlth Off, Nai Mueang, Nakhon Phanom, Thailand

24.Changi Gen Hosp, Dept Lab Med, Singapore, Singapore

25.Tan Tock Seng Hosp, Mol Biol Lab, Singapore, Singapore

26.Natl Hosp Obstet & Gynaecol, Hanoi, Vietnam

27.Angkor Hosp Children, Cambodia Oxford Med Res Unit, Siem Reap, Cambodia

28.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou, Guangdong, Peoples R China

29.Ctr Dis Control & Prevent, Div Global Hlth Protect, Atlanta, GA USA

30.Genome Inst Singapore, Infect Dis Grp, Singapore, Singapore

期刊名称：PLOS NEGLECTED TROPICAL DISEASES （ 影响因子：4.411； 五年影响因子：4.898 ）

ISSN： 1935-2735

年卷期： 2019 年 13 卷 6 期

页码：

收录情况： SCI

摘要： Background In 2015, Singapore had the first and only reported foodborne outbreak of invasive disease caused by the group B Streptococcus (GBS; Streptococcus agalactiae). Disease, predominantly septic arthritis and meningitis, was associated with sequence type (ST)283, acquired from eating raw farmed freshwater fish. Although GBS sepsis is well-described in neonates and older adults with co-morbidities, this outbreak affected non-pregnant and younger adults with fewer co-morbidities, suggesting greater virulence. Before 2015 ST283 had only been reported from twenty humans in Hong Kong and two in France, and from one fish in Thailand. We hypothesised that ST283 was causing region-wide infection in Southeast Asia. Methodology/Principal findings We performed a literature review, whole genome sequencing on 145 GBS isolates collected from six Southeast Asian countries, and phylogenetic analysis on 7,468 GBS sequences including 227 variants of ST283 from humans and animals. Although almost absent outside Asia, ST283 was found in all invasive Asian collections analysed, from 1995 to 2017. It accounted for 29/38 (76%) human isolates in Lao PDR, 102/139 (73%) in Thailand, 4/13 (31%) in Vietnam, and 167/739 (23%) in Singapore. ST283 and its variants were found in 62/62 (100%) tilapia from 14 outbreak sites in Malaysia and Vietnam, in seven fish species in Singapore markets, and a diseased frog in China. Conclusions GBS ST283 is widespread in Southeast Asia, where it accounts for a large proportion of bacteraemic GBS, and causes disease and economic loss in aquaculture. If human ST283 is fishborne, as in the Singapore outbreak, then GBS sepsis in Thailand and Lao PDR is predominantly a foodborne disease. However, whether transmission is from aquaculture to humans, or vice versa, or involves an unidentified reservoir remains unknown. Creation of cross-border collaborations in human and animal health are needed to complete the epidemiological picture. Author summary An outbreak due to a bacterium called Streptococccus agalactiae in Singapore in 2015 was caused by a clone called ST283, and was associated with consumption of raw freshwater-fish. It was considered unique as it was the only reported foodborne outbreak of this bacterium. Our new data show that invasive ST283 disease is far from unique. ST283 has been causing disease in humans and farmed fish in SE Asian countries for decades. Reports of ST283 are almost absent outside Asia. We suspect that human ST283 is fishborne in other Asian countries, as it was in Singapore, but we haven't looked at this yet. We don't know where ST283 originally came from; it may have been transmitted from humans to fish, or come from another animal. More studies are needed to determine ST283's geographical extent and burden of disease, as well as its origin, how it is transmitted, and what enables it to be so aggressive. We may then be able to interrupt transmission, to the benefit of fish, farmers, and the general public.