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Determination of Piperazine in Eggs Using Accelerated Solvent Extraction (ASE) and Solid Phase Extraction (SPE) with High-Performance Liquid Chromatography - Fluorescence Detection (HPLC-FLD) and Pre-Column Derivatization with Dansyl Chloride

文献类型： 外文期刊

作者： Bu, Xiaona 1 ; Pang, Maoda 3 ; Wang, Bo 2 ; Zhang, Yangyang 1 ; Xie, Kaizhou 1 ; Zhao, Xia 1 ; Wang, Yajuan 1 ; Guo, Yawe 1 ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China

2.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr,Minist Sci & Technol, Key Lab Control Technol & Stand Agroprod & Qua, Lab Food Qual & Safety,State Key Lab Cultivat Bas, Nanjing, Peoples R China

4.Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China

关键词： Accelerated solvent extraction (ASE); dansyl chloride; high-performance liquid chromatography - fluorescence detection (HPLC-FLD); piperazine; solid phase extraction (SPE)

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

ISSN： 0003-2719

年卷期：

页码：

收录情况： SCI

摘要： A high-performance liquid chromatography - fluorescence detection (HPLC-FLD) method was established for the determination of piperazine residues in whole eggs, albumen, and yolk employing pre-column derivatization with dansyl chloride. The egg samples were treated by accelerated solvent extraction (ASE) and the analytes purified by solid phase extraction (SPE). Acetonitrile/ultrapure water was used as the mobile phase for gradient elution. The linear dynamic range was from 6.80 to 200.0 mu g/kg for whole eggs, 7.50 to 200.0 mu g/kg for albumen, and 6.50 to 200.0 mu g/kg for yolk with coefficients of determination (R-2) greater than or equal to 0.9992. The average recoveries of piperazine from egg samples were from 72.86 to 89.26% when the concentrations were equal to the limit of quantification, 0.5 times the maximum residue limit, equal to the maximum residue limit, and twice the maximum residue limits. The relative standard deviations were between 1.73 and 4.99%. The limits of detection of piperazine in eggs were from 1.92 to 2.50 mu g/kg, and the limits of quantification were between 6.50 and 7.50 mu g/kg. The method meets the requirements of the European Union, the Ministry of Agriculture of the People's Republic of China and the U.S. Food and Drug Administration (FDA) for the determination of veterinary drug residues. The validated HPLC-FLD method was successfully applied to the determination of piperazine residues in 50 eggs purchased from local supermarkets.

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