Identification and application of markers closely linked to the restorer gene (Rfm) in rapeseed (Brassica napus L.)
文献类型： 外文期刊
作者： Long, Weihua 1 ; Hu, Maolong 1 ; Gao, Jianqin 1 ; Sun, Lijie 1 ; Zhang, Jiefu 1 ; Pu, Huiming 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Cotton & Rapeseed Nanjing, Minist Agr, Inst Ind Crops, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China
关键词： restorer gene; MI CMS; rapeseed (Brassica napus L.); near-isogenic lines (NILs); marker-assisted selection (MAS)
期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）
ISSN： 1344-7610
年卷期： 2019 年 69 卷 2 期
页码：
收录情况： SCI
摘要： The Mutsu-Isuzu cytoplasmic male sterility (MI CMS) system is one of the three-line hybrid systems used in China. As we know, the hybrid system is tightly associated with the yield variation in F-1 heterosis, while the restorer gene for the MI CMS (Rfm) has not been finely mapped for further application in marker-assisted selection (MAS). In this study, the sets of near-isogenic lines (NILs) of Rfm in two different genetic backgrounds were hybridized with the genome-wide 60 K single-nucleotide polymorphism (SNP) chip of Brassica for screening the possible associated genomic region of Rfm. Through screening genotypes with SNP loci and sequencing the candidate loci, one 2.5 Mb physical region (covering three scaffolds) on chrA09 was identified as the candidate for the Rfm region. Then, the SSR markers for the target scaffolds were used to detect the recombination in an F-2 population and narrowed the Rfm gene within the genetic distance of 0.52 cM, equivalent to a 350 kb physical segment. Moreover, the markers were tested to improve new elite restoration lines and to assess the percentage of hybrid seeds. Our results could potentially accelerate the map-based cloning of the Rfm gene to benefit rapeseed breeding.
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