Structural elucidation and immunomodulatory activity of a beta-D-glucan prepared by freeze-thawing from Hericium erinaceus
文献类型： 外文期刊
作者： Wu, Di 1 ; Tang, Chuan 1 ; Liu, Yanfang 1 ; Li, Qiaozhen 1 ; Wang, Wenhan 1 ; Zhou, Shuai 1 ; Zhang, Zhong 1 ; Cui, Fengj 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Key Lab Edible Fungi Resources & Utilizat South, Inst Edible Fungi,Minist Agr, Shanghai 201403, Peoples R China
2.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
关键词： Hericium erinaceus; beta-D-glucan; Structural analysis; Immunomodulation activity
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2019 年 222 卷
页码：
收录情况： SCI
摘要： H6PC20, a water-soluble beta-D-glucan, with a molecular mass of 2390 kDa (HPSEC-MALLS-RI), was obtained by extracting the fresh fruiting bodies of Hericium erinaceus at mature stage with hot water, freeze-thawing, precipitating and further rinsing with 20% (v/v) of ethanol. The primary structure of H6PC20 was proposed as a beta-(1 -> 3)-linked-D-glucan with a (1 -> 6)-beta-linked-D-glucopyranosyl side-branching unit on every third residue by using methylation analysis, H-1 and C-13 NMR spectroscopy, including COSY, TOCSY, HSQC and HMBC. The rho value (R-g/R-h) and the exponent (alpha) of [eta] = k M-w(alpha) of H6PC20 were calculated as 1.86 and 0.54, respectively, indicating flexible random coil conformation of H6PC20 in aqueous solution. H6PC20 could induce the proliferation of lymphocytes and enhance the expression of inflammatory cytokines IL-6, TNF-alpha and IL-1 beta secreted by THP-1 macrophages. The present study indicated that the macromolecular beta-glucan H6PC20 possessed potent immune-enhancing bioactivities and could be a potential ingredient for healthy food or medicine development.
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