Tissue expression and bioinformatics analysis of the vitellogenin gene of Asian arowana (Scleropages formosus)
文献类型： 外文期刊
作者： Pan, Xianhui 1 ; Liu, Yi 1 ; Zhou, Kangqi 1 ; Mu, Xidong 1 ; Zheng, Shuming 3 ; Liu, Chao 1 ; Hu, Yinchang 1 ;
作者机构： 1.CAFS, Pearl River Fisheries Res Inst, Guangdong Modern Leisure Fisheries Engn Technol C, Minist Agr,Key Lab Recreat Fisheries, Guangzhou, Guangdong, Peoples R China
2.Guangxi Inst Fisheries, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Nanning, Peoples R China
3.China Southwest Univ, Chongqing, Peoples R China
关键词： cloning; Scleropages formosus; tissue expression; vitellogenin
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN： 0175-8659
年卷期： 2019 年 35 卷 4 期
页码：
收录情况： SCI
摘要： The RACE technique was used to clone the full-length vitellogenin (VTG) cDNA sequence of Asian arowana (Scleropages formosus). The full-length sequence was 5,550 bp with an open reading frame of 5,238 bp, encoding 1,745 amino acids, and 5 ' and 3 ' UTRs (untranslated regions) of 45 bp and 267 bp, respectively. Phylogenetic analysis showed that S. formosus and silver arowana (Osteoglossum bicirrhosum) share a close evolutionary relationship (bootstrap 100%). The quantitative real-time PCR results showed that vtg expression was significantly higher in liver and gonads of male and female fish compared with its expression in the other tissues tested (p < 0.01). The relative expression levels of vtg in liver, gland, kidney, heart, head kidney, and brain of female fish were significantly higher than in the corresponding tissues of male fish (p < 0.05).
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