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Transcriptomic analysis provides insight into the mechanism of salinity adjustment in swimming crab Portunus trituberculatus

文献类型： 外文期刊

作者： Gao, Baoquan 1 ; Sun, Dongfang 1 ; Lv, Jianjian 1 ; Ren, Xianyun 1 ; Liu, Ping 1 ; Li, Jian 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Shandong, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China

关键词： Transcriptomics; Salinity adjustment; Swimming crab; Portunus trituberculatus

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期： 2019 年 41 卷 8 期

页码：

收录情况： SCI

摘要： BackgroundLow salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the swimming crab (Portunus trituberculatus) is adaptive to relatively low salinity. However, the mechanisms underlying salinity stress responses in P. trituberculatus is not very clear.ObjectivesThe primary objective of this study was to describe the salinity adaptation mechanism in P. trituberculatus.MethodsThe crabs were exposed to low salinity stress, and gill tissue was sampled at 0, 12, 36, 48 and 72h and subjected to high throughput sequencing. Subsequently, we tested the accuracy and quality of the sequencing results, and then carried out GO and KEGG bioinformatics on the differentially expressed genes (DEG).ResultsEach sample yielded more than 1.1Gb of clean data and 23 million clean reads. The process was divided into early (0-12h), middle (12-48h), and late phase (48-72h). A total of 1971 (1373 up-regulated, 598 down-regulated), 1212 (364 up-regulated, 848 down-regulated), and 555 (187 up-regulated, 368 down-regulated) DEGs with annotations were identified during the three stages, respectively. DEGs were mainly associated with lipid metabolism energy metabolism, and signal transduction from the three stages, respectively.ConclusionA substantial number of genes were modified by salinity stress, along with a few important salinity acclimation pathways. This work provides valuable information on the salinity adaptation mechanism in P. trituberculatus. In addition, the comprehensive transcript sequences reported in this study provide a rich resource for identification of novel genes in this and other crab species.

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