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The OsmiR396-OsGRF8-OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa)

文献类型： 外文期刊

作者： Dai, Zhengyan 1 ; Tan, Jiang 1 ; Zhou, Cong 2 ; Yang, Xiaofang 1 ; Yang, Fang 2 ; Zhang, Shijuan 4 ; Sun, Shichen 5 ; Mia 1 ;

作者机构： 1.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Key Lab Insect Dev & Evolutionary Biol, Shanghai, Peoples R China

2.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Hubei, Peoples R China

3.Univ Chinese Acad Sci, Shanghai, Peoples R China

4.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China

5.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Tillage, Haerbin, Peoples R China

6.Chinese Acad Sci, Northern Japon Rice Mol Breeding Joint Res Ctr, Haerbin, Peoples R China

关键词： OsmiR396; brown planthopper; flavonoid; rice (Oryza sativa); OsF3H

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2019 年 17 卷 8 期

页码：

收录情况： SCI

摘要： Multi-functional microRNAs (miRNAs) are emerging as key modulators of plant-pathogen interactions. Although the involvement of some miRNAs in plant-insect interactions has been revealed, the underlying mechanisms are still elusive. The brown planthopper (BPH) is the most notorious rice (Oryza sativa)-specific insect that causes severe yield losses each year and requires urgent biological control. To reveal the miRNAs involved in rice-BPH interactions, we performed miRNA sequencing and identified BPH-responsive OsmiR396. Sequestering OsmiR396 by overexpressing target mimicry (MIM396) in three genetic backgrounds indicated that OsmiR396 negatively regulated BPH resistance. Overexpression of one BPH-responsive target gene of OsmiR396, growth regulating factor 8 (OsGRF8), showed resistance to BPH. Furthermore, the flavonoid contents increased in both the OsmiR396-sequestered and the OsGRF8 overexpressing plants. By analysing 39 natural rice varieties, the elevated flavonoid contents were found to correlate with enhanced BPH resistance. Artificial applications of flavonoids to wild type (WT) plants also increased resistance to BPH. A BPH-responsive flavanone 3-hydroxylase (OsF3H) gene in the flavonoid biosynthetic pathway was proved to be directly regulated by OsGRF8. A genetic functional analysis of OsF3H revealed its positive role in mediating both the flavonoid contents and BPH resistance. And analysis of the genetic correlation between OsmiR396 and OsF3H showed that down-regulation of OsF3H complemented the BPH resistance characteristic and simultaneously decreased the flavonoid contents of the MIM396 plants. Thus, we revealed a new BPH resistance mechanism mediated by the OsmiR396-OsGRF8-OsF3H-flavonoid pathway. Our study suggests potential applications of miRNAs in BPH resistance breeding.

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