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Newly identified APN splice isoforms suggest novel splicing mechanisms may underlie circRNA circularization in moth

文献类型： 外文期刊

作者： Gao, Meijing 1 ; Liu, Yuan 1 ; Wang, Yun 3 ; Zhang, Xiao 1 ; Dong, Sa 4 ; Liu, Xianjin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China

3.Jinling Inst Technol, Hort Dept, Nanjing, Jiangsu, Peoples R China

4.Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou, Jiangsu, Peoples R China

关键词： alternative splicing; aminopeptidases N; circular RNA; splicing mechanism

期刊名称：FEBS OPEN BIO （ 2020影响因子：2.693； 五年影响因子：2.519 ）

ISSN： 2211-5463

年卷期：

页码：

收录情况： SCI

摘要： Circular RNA (circRNA) have long been considered by-products of splicing errors, but the coordination of RNA transcription and exon circularization events remains poorly understood. Here, we investigated this question using genes encoding aminopeptidases N (APNs), which are receptors of Bacillus thuringiensis toxins, in the cotton bollworm, Helicoverpa armigera. We cloned and sequenced the cDNA of ten APN genes (HaAPN1-10) located in the same APN gene cluster, and detected 20 and 14 novel splicing isoforms with exon skipping in HaAPN1 and HaAPN3, respectively, whereas no or very few variants were found in the remaining genes. Further study identified 14 and 6 circular RNA (circRNA) in HaAPN1 and HaAPN3, respectively. Neither novel splicing isoforms nor circRNA were detected in HaAPN2 and HaAPN5. Distinct from the conventional GT/AG splicing signal, short co-directional repeats were involved in the splicing of the linear and circular isoforms of HaAPN1 and HaAPN3. Identification of the splice sites revealed that the linear isoforms may be related in some way to the circularization. Moreover, phylogenetic analysis and detection of circRNA of the APN gene of the diamondback moth, Plutella xylostella (PxAPN3), showed that circRNA formation is relatively conserved during the lepidopteran evolutionary process. These results contribute to an improved understanding of lepidopteran APNs and this novel class of insect circRNA.

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