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Enteropathogenic Escherichia coli is a predominant pathotype in healthy pigs in Hubei Province of China

文献类型： 外文期刊

作者： Zhuo, Wenxiao 1 ; Zhao, Yang 1 ; Zhao, Xianglin 1 ; Yao, Zhiming 1 ; Qiu, Xiuxiu 1 ; Huang, Yaxue 1 ; Li, Huaixia 1 ; Shen, Jing 1 ; Zhu, Zhihao 1 ; Li, Tingting 2 ; Li, Shao W. En 1 ; Qi Huang 1 ; Zhou, Rui 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Hubei Anim Dis Prevent & Control Ctr, Wuhan 430070, Peoples R China

3.Coll Vet Med, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Peoples R China

4.Minist Sci Technol China, Coll Vet Med, Int Res Ctr Anim Dis, Wuhan 430070, Peoples R China

5.HZAU HVSEN Res Inst, Wuhan 430042, Peoples R China

关键词： Intestinal pathogenic Escherichia coli (InPEC); enteropathogenic E. coli (EPEC); healthy pig; prevalence; in silico typing; pathogenicity

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.0； 五年影响因子：4.3 ）

ISSN： 1364-5072

年卷期： 2023 年 134 卷 11 期

页码：

收录情况： SCI

摘要： Aim: This study aims to investigate the prevalence of intestinal pathogenic Escherichia coli (InPEC) in healthy pig-related samples and evaluate the potential virulence of the InPEC strains. Methods and results: A multiplex PCR method was established to identify different pathotypes of InPEC. A total of 800 rectal swab samples and 296 pork samples were collected from pig farms and slaughterhouses in Hubei province, China. From these samples, a total of 21 InPEC strains were isolated, including 19 enteropathogenic E. coli (EPEC) and 2 shiga toxin-producing E. coli (STEC) strains. By whole-genome sequencing and in silico typing, it was shown that the sequence types and serotypes were diverse among the strains. Antimicrobial susceptibility assays showed that 90.48% of the strains were multi-drug resistant. The virulence of the strains was first evaluated using the Galleria mellonella larvae model, which showed that most of the strains possessed medium to high pathogenicity. A moderately virulent EPEC isolate was further selected to characterize its pathogenicity using a mouse model, which suggested that it could cause significant diarrhea. Bioluminescence imaging (BLI) was then used to investigate the colonization dynamics of this EPEC isolate, which showed that the EPEC strain could colonize the mouse cecum for up to 5 days.

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