文献类型： 外文期刊
作者： Li, Shikai 1 ; Wang, Wei 1 ; Yang, Mingju 1 ; Huang, Fujiang 1 ; Wang, Xue 1 ; Zeng, Sheng 1 ; Xiang, Yan 1 ; Zhou, Lu 1 ;
作者机构： 1.Guizhou Acad Agr Sci, Fisheries Res Inst, 2448 Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China
2.Engn & Technol Ctr Special Fisheries, 2448 Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China
关键词： Semilabeo obscurus; SNP; Molecular marker; Germplasm conservation
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.991； 五年影响因子：0.897 ）
ISSN： 1877-7252
年卷期：
页码：
收录情况： SCI
摘要： Semilabeo obscurus, as an endangered species, is a certain economic fish in China and Vietnam. However, study on its genetic diversity is still lacking. In this study, 168 candidate SNPs were verified by Sanger-sequencing. Of these, 123 polymorphic SNP markers were successfully genotyped. The observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information conten (PIC) ranged from 0.0968-0.9310 (mean 0.3590), 0.0936-0.5085 (mean 0.3550) and 0.0879-0.3750 (mean 0.2807), respectively. Only 3 SNPs (Sob-016, Sob-050 and Sob-115) were significantly deviated from Hardy-Weinberg equilibrium (P < 0.05). Our study may help researchers better understand the genetic traits of S. obscurus,and provide reference for further protecting and reasonably utilizing its genetic resources.
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