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Genetic diversity and population structure analysis in a large collection of Vicia amoena in China with newly developed SSR markers

文献类型： 外文期刊

作者： Wu, Feifei 1 ; Zhang, Shangxiong 1 ; Gao, Qiu 2 ; Liu, Fang 2 ; Wang, Jianli 3 ; Wang, Xianguo 1 ;

作者机构： 1.China Agr Univ, Coll Grassland Sci & Technol, Beijing 100193, Peoples R China

2.Natl Herbage Germplasm Conservat Ctr China, Beijing 10025, Peoples R China

3.Heilongjiang Acad Agr Sci, Grass & Sci Inst, Harbin 150086, Peoples R China

关键词： Vicia amoena; SSR development; genetic variation; population structure

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Vicia amoena is a high-nutritional quality forage similar to alfalfa. However, studies on the genetic background of V. amoena are scarce. In the present study, the genetic variation of 24 V. amoena populations was assessed with newly developed simple sequence repeat (SSR) markers. A total of 8799 SSRs were identified in the V. amoena genomic-enriched sequences, and the most abundant repeat number was four. A total of 569 sampled individuals were assayed to evaluate the genetic diversity of the V. amoena populations based on 21 polymorphic SSR primers. The polymorphism information content (PIC) ranged from 0.896 to 0.968, with an average of 0.931, which indicated that the markers were highly informative. Based on analysis of molecular variance, 88% of the variance occurred within populations, and the remaining 12% of the variance occurred among populations. The high degree of gene flow (Nm= 4.958) also showed slight differentiation among the V. amoena populations. The V. amoena populations were mainly clustered by steppe and mountain habitats based on principal coordinate analysis (PCoA) and STRUCTURE analysis. This indicated that the elevation and special habitat of geographical origins may be important factors affecting the clustered pattern of V. amoena populations. Neighbour-joining (NJ) analysis did not separate the populations well by geographical origin, which indicated that the genetic structure of V. amoena was complex and needs further study. Overall, our results showed that the newly developed SSR markers could benefit the V. amoena research community by providing genetic background information to help establish a foundation for breeding improvement and germplasm resource conservation.

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