文献类型： 外文期刊
作者： Qin, Ting 1 ; Dowah, Richline Gedeh Wentee 1 ; Chen, Kai 2 ; Xi, Bingwen 1 ; Pan, Liangkun 2 ; Xie, Jun 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Integrated Rice Fish Farming Ecol, Minist Agr & Rural Affairs, Wuxi 214081, Peoples R China
3.Univ Liberia, Fac Aquaculture, Dept Fisheries & Aquaculture Sci, Monrovia, Liberia
关键词： Carvacrol; Edwardsiella piscicida; Biofilm formation; Hemolytic activity; Anti-virulence
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）
ISSN： 0882-4010
年卷期： 2024 年 196 卷
页码：
收录情况： SCI
摘要： With the alarming rise of antibiotic-resistant bacteria, novel antibacterial substances are urgently needed for controlling and treating multidrug-resistant bacterial infections. Edwardsiella piscicida is an important zoonotic enteric pathogen, that can cause systemic hemorrhagic septicemia in fish. Carvacrol, a major terpene of oregano essential oil, has a wide range of antibacterial activities. This study aimed to analyze the effect of carvacrol on the growth and virulence of E. piscicida in vitro. . The minimum inhibitory concentration (MIC) of carvacrol against E. piscicida was 125 mu g/mL. The sub-inhibitory concentrations of carvacrol significantly decreased the biofilm formation of E. piscicida in a dose dependent manner, whereas increased the hemolytic activity with a negative correlation. The quantitative real-time PCR results showed that carvacrol at sub-MICs downregulated the expression of related virulence genes, including flagellum ( fimA , fliC, flgN), ), hemolysins ( ethA, ethB), ), quorum sensing systems ( luxR, qseB), ), T3SS ( esrB, esrC) ) and T6SS ( evpB, evpC). ). Moreover, carvacrol (<= 1/8 <= 1/8 MIC) reduced the cytotoxicity, adherence and internalization activities of E. piscicida to the EPC cells. In vivo trial, the diet mixed with carvacrol increased the survival of zebrafish infected with E. piscicida. . Overall, these findings suggested that carvacrol might be a promising therapeutic agent against E. piscicida infection in aquaculture.
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