文献类型： 外文期刊
作者： Xing, Yujun 1 ; Liang, Jie 2 ; Dong, Fei 2 ; Wu, Jirong 2 ; Shi, Jianrong 2 ; Xu, Jianhong 1 ; Wang, Jinke 3 ;
作者机构： 1.Jiangsu Acad Agr Sci, Minist Sci & Technol, Inst Food Safety & Nutr, Collaborat Innovat Ctr Modern Grain Circulat & Saf, Nanjing 210014, Peoples R China
2.Jiangsu Acad Agr, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China
3.Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing 210096, Peoples R China
期刊名称：ACS OMEGA （ 影响因子：4.1； 五年影响因子：4.0 ）
ISSN： 2470-1343
年卷期： 2023 年 8 卷 32 期
页码：
收录情况： SCI
摘要： We developed a novel loop-mediated isothermal amplification(LAMP)method using DNA captured on polyacrylamide microparticles (PAMMPs)as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection ofgenetically modified organisms (GMOs). Here, DNA was extracted bya fast and cost-effective method using PAMMPs. Four LAMP primers weredesigned for the PAMMPs@DNA-LAMP method to detect the cauliflowermosaic virus 35S (CaMV35S) promotor in GMOs. We thus developed thismethod for rapid extraction of DNA (5-10 min) and fast amplificationof DNA within & SIM;30 min at a constant temperature of 63 & DEG;C.Moreover, the DNA captured by PAMMPs (PAMMPs@DNA) could be effectivelydetected by both conventional and quantitative PCR (qPCR) and LAMP.The PAMMPs@DNA-LAMP method was validated with high specificity, sensitivity,and performance for practical sample analysis. This assay detected0.01% target sequences, which had a high specificity like qPCR andbetter than the conventional PCR (cPCR). Furthermore, PAMMPs@DNA-LAMPwas successfully used to extract and detect DNA from food samplesof the major crops (soybean, maize, rice, etc.). In summary, a novelPAMMPs@DNA-LAMP assay has been developed, which has higher sensitivityand spends less time than the cPCR detection using the conventionalDNA extracted process. This method offers a novel approach for rapiddetection of GMOs in the field.
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