De Novo Assembly, Characterization, and Comparative Transcriptome Analysis of Mature Male and Female Gonads of Rabbitfish (Siganus oramin) (Bloch & Schneider, 1801)
文献类型： 外文期刊
作者： Huang, Xiaolin 1 ; Huang, Zhong 1 ; Li, Qiang 3 ; Li, Wenjun 3 ; Han, Chong 3 ; Yang, Yukai 1 ; Lin, Heizhao 1 ; Wu, Qiaer 1 ; Zhou, Yanbo 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr, Guangzhou 510300, Peoples R China
2.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Natl Fishery Resources & Environm Dapeng Observat, Shenzhen 518121, Peoples R China
3.Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China
关键词： rabbitfish; transcriptome; gonad; reproduction-related genes
期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2024 年 14 卷 9 期
页码：
收录情况： SCI
摘要： The rabbitfish, Siganus oramin, is a commercially important table fish in southeastern China. However, there have been few studies on its gonad development and reproduction regulation. Comparative transcriptome analysis was first performed on adult male and female gonads of S. oramin. In total, 47,070 unigenes were successfully assembled and 22,737 unigenes were successfully annotated. Through comparative transcriptome analysis of male and female gonads, a total of 6722 differentially expressed genes were successfully identified, with 3528 upregulated genes and 3154 downregulated genes in the testes. In addition, 39 differentially expressed reproduction-related genes were identified. Finally, quantitative real-time PCR was used to validate the expression levels of several differentially expressed genes. These results provide important data for further studying the function of reproduction-related genes and the molecular mechanism regulating gonad development and reproduction in S. oramin.
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