The critical role of CmCIPK1-CmRbohD1/D2 complexes in generating H2O2 signals for enhancing salt tolerance in pumpkin
文献类型： 外文期刊
作者： Wei, Lanxing 1 ; Xu, Dandi 1 ; Zhou, Lijian 1 ; Chen, Hui 1 ; Peng, Zhaowen 1 ; Chen, Guoyu 1 ; Wang, Lihui 1 ; Cao, Haishun 1 ; Peng, Yuquan 1 ; Geng, Shouyu 1 ; Bie, Zhilong 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Hubei, Peoples R China
2.Hubei Hongshan Lab, Wuhan 430070, Hubei, Peoples R China
3.Guangdong Acad Agr Sci, Inst Facil Agr, Guangzhou 510640, Guangdong, Peoples R China
关键词： Pumpkin; Rboh; Root transformation; Salinity; Protein interaction
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）
ISSN： 2095-9885
年卷期： 2025 年 11 卷 2 期
页码：
收录情况： SCI
摘要： Pumpkin is of great economic importance not only as food resources but also as the most widely used rootstock to graft cucurbit crops. reactive oxygen species (ROS) burst, which further enhances Na+ export and K+ uptake. RbohD activation requires calcium signaling. However, the underlying mechanism remains largely unknown. In this study, we discovered two RbohD members from pumpkin involved in the ROS burst at the early stage of salt stress. CmRbohD1 and CmRbohD2 were functionally redundant and double mutation significantly impaired salt tolerance in pumpkin. Overexpression of CmRbohD1 and CmRbohD2 mitigated salinity-induced damage and maintained a relatively low Na+ content and high K+ content. We screened the potential calcineurin B-like interacting protein kinases (CmCIPKs) which could bind with CmRbohD1 and CmRbohD2. Our results revealed that two RbohDs formed complexes specifically with CmCIPK1, thereby bursting ROS production. Overexpression of CmCIPK1 promoted the early ROS burst under salt stress condition and maintained a relatively balanced Na+/K+ homeostasis. Altogether, we proposed a CmCIPK1-CmRbohD1/D2 complex for pumpkin salt stress signaling transduction, which regulates the ROS burst and Na+/K+ homeostasis. Our findings offer unprecedented insights into the general mechanism of pumpkin salt tolerance.
- 相关文献
作者其他论文 更多>>
-
Identification of the G2-like transcriptional factor gene family reveals their critical roles in controlling chlorophyll accumulation of melon fruit
作者：Cao, Haishun;Wu, Tingquan;Zhang, Changyuan;Shi, Liangliang;Gong, Mingxi;Anwar, Ali;Song, Shiwei
关键词：G2-like transcription factors; Melon; Chlorophyll; Fruit peel; GLKs; APRR2
-
Genome-wide identification and expression analysis of the cryptochromes reveal the CsCRY1 role under low-light-stress in cucumber
作者：Cao, Haishun;Wang, Rui;Zhao, Junhong;Shi, Liangliang;Wu, Tingquan;Zhang, Changyuan;Huang, Yuan
关键词：cryptochromes; cucumber; low-light-stress; photoreceptors; alternative splicing
-
Transcriptomic and functional characterization reveals CsHAK5;3 as a key player in K plus homeostasis in grafted cucumbers under saline conditions
作者：Peng, Yuquan;Cao, Haishun;Peng, Zhaowen;Zhou, Lijian;Sohail, Hamza;Cui, Lvjun;Yang, Li;Huang, Yuan;Bie, Zhilong;Cao, Haishun
关键词：Grafted cucumber; Salinity; K plus homeostasis; HAK5; Transcriptome
-
CmoNAC1 in pumpkin rootstocks improves salt tolerance of grafted cucumbers by binding to the promoters of CmoRBOHD1, CmoNCED6, CmoAKT1;2 and CmoHKT1;1 to regulate H2O2, ABA signaling and K+/Na+ homeostasis
作者：Peng, Yuquan;Cao, Haishun;Cui, Lvjun;Wang, Ying;Wei, Lanxing;Geng, Shouyu;Yang, Li;Huang, Yuan;Bie, Zhilong;Cao, Haishun;Bie, Zhilong
关键词：
-
An efficient root transformation system for CRISPR/Cas9-based analyses of shoot-root communication in cucurbit crops
作者：Geng, Shouyu;Sohail, Hamza;Cao, Haishun;Sun, Jingyu;Chen, Zhi;Zhou, Lijian;Wang, Wenbo;Ye, Runwen;Yang, Li;Bie, Zhilong;Cao, Haishun
关键词：
-
ESCO2 promotes lung adenocarcinoma progression by regulating hnRNPA1 acetylation
作者：Zhu, Hui-er;Chen, De-xiong;Li, Tao;Shi, Shengnan;Chen, Weiping;Chen, Hui
关键词：Acetylation; Metabolism reprogramming; Lung adenocarcinoma; ESCO2; hnRNPA1
-
Overseas immigration of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), invading Korea and Japan in 2019
作者：Wu, Ming-Fei;Chen, Hui;Ma, Jian;Hu, Gao;Qi, Guo-Jun;Liu, Jie;Jiang, Yu-Ying;Lee, Gwan-Seok;Otuka, Akira
关键词：Asian migration arena; atmospheric circulation; Spodoptera frugiperda; trajectory analysis windborne insect migration
