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A simple, rapid and effective method for total RNA extraction from Lentinula edodes

文献类型: 外文期刊

作者: Li, Jun-hui 1 ; Tang, Chuan-hong 2 ; Song, Chun-yan 2 ; Chen, Ming-jie 2 ; Feng, Zhi-yong 2 ; Pan, Ying-jie 3 ;

作者机构: 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China; Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China; Shanghai Fisheries Univ, Coll Food Sci, Shanghai 200090, Peoples R China

3.Shanghai Acad Agr Sci, Inst Edible Fungi, Shangh

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

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收录情况: SCI

摘要: A rapid, inexpensive and reliable method for total RNA extraction from fruiting bodies of Lentinula edodes containing large quantities of polysaccharides and secondary metabolites is described. An initial extraction step using saturated NaCl solution facilitates the separation of nucleic acids from contaminants and, after further extraction with organic solvents and precipitation with 2-propanol, total RNA of high purity and suitable for applications such as cDNA synthesis, RT-PCR and Northern blot hybridization was obtained. The procedure may also have wider applicability for total RNA extraction from the tissues of other mushrooms.

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