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Radical thermal therapy against infection with decapod iridescent virus 1 (DIV1)

文献类型： 外文期刊

作者： Guo, Xiao-Meng 1 ; Qiu, Liang 1 ; Gao, Wen 1 ; Wang, Guo-Hao 1 ; Chen, Xing 1 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc,Pilot Na, Minist Agr & Rural Affairs,Qingdao Key Lab Maricul, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Shanghai 201306, Peoples R China

3.Network Aquaculture Ctr Asia Pacific, Bangkok 10900, Thailand

4.Network Aquaculture Ctr Asia Pacific, POB 1040,Kasetsart PO, Bangkok 10900, Thailand

关键词： Infection with decapod iridescent virus 1; (iDIV1); Thermal treatment; Temperature restoration; Radical cure; Penaeus vannamei

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 561 卷

页码：

收录情况： SCI

摘要： Decapod iridescent virus 1 (DIV1) is a newly found double-stranded DNA virus that can cause huge losses to the aquaculture industry. The potential of thermal therapy to treat and cure the disease, namely infection with DIV1 (iDIV1), in white leg shrimp Penaeus vannamei, was evaluated in this study with the thermal treatment and temperature restoration (TT/TR) protocol. After being challenged with DIV1 strain SHIV20141215 via intramuscular injection, the shrimp in different groups received a 15-day TT from 28 degrees C to 38 degrees C, respectively, and then 15-day TR at 28 degrees C. All shrimp in groups with TT below 32 degrees C died rapidly within 5 d post-infection (dpi) with confirmatory iDIV1 by TaqMan qPCR, histopathological H&E staining and ISDL, and cytopathological TEM. The development of iDIV1 was suppressed at 34 degrees C but revived after TT to 28 degrees C. Elimination of DIV1 at 36 degrees C was confirmed with the same survival curve of the unchallenged control group, no replication of DIV1 DNA copies, and negative results of clinical signs, histopathology, ISDL, and TEM during the TT/TR process. The reproducibility validation of TT/TR at 36 degrees C verified the elimination of DIV1 from the challenged shrimp. This is the first report of radical thermal therapy from the emerging disease iDIV1 using TT at 36 degrees C, which provides an innovative measure to cure the fatal viral infection, especially for the larvae and broodstock of shrimp varieties with special genetic properties.

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