Improvement of Rice Blast Resistance in TGMS Line HD9802S through Optimized Anther Culture and Molecular Marker-Assisted Selection
文献类型： 外文期刊
作者： Chen, Pingli 1 ; Gao, Guanjun 1 ; Lou, Guangming 1 ; Hu, Jie 1 ; Wang, Yufu 1 ; Liu, Rongjia 1 ; Zhao, Da 1 ; Liu, Qing 2 ; Sun, Bingrui 2 ; Mao, Xingxue 2 ; Jiang, Liqun 2 ; Zhang, Jing 2 ; Lv, Shuwei 2 ; Yu, Hang 2 ; Chen, Wenfeng 2 ; Fan, Zhilan 2 ; Li, Chen 2 ; He, Yuqing 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan 430070, Peoples R China
2.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Key Lab New Technol Rice Breeding, Guangzhou 510640, Peoples R China
3.Minist Agr & Rural Affairs, Guangdong Key Lab Genet & Breeding High Qual Rice, Guangzhou 510640, Peoples R China
关键词： rice blast resistance; thermosensitive genic male sterile line; anther culture; marker-assisted selection
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 19 期
页码：
收录情况： SCI
摘要： Rice blast caused by Magnaporthe oryzae is one of the most serious rice diseases worldwide. The early indica rice thermosensitive genic male sterile (TGMS) line HD9802S has the characteristics of stable fertility, reproducibility, a high outcrossing rate, excellent rice quality, and strong combining ability. However, this line exhibits poor blast resistance and is highly susceptible to leaf and neck blasts. In this study, backcross introduction, molecular marker-assisted selection, gene chipping, anther culture, and resistance identification in the field were used to introduce the broad-spectrum blast-resistance gene R6 into HD9802S to improve its rice blast resistance. Six induction media were prepared by varying the content of each component in the culture medium. Murashige and Skoog's medium with 3 mg/L 2,4-dichlorophenoxyacetic acid, 2 mg/L 1-naphthaleneacetic acid, and 1 mg/L kinetin and N6 medium with 800 mg/L casein hydrolysate, 600 mg/L proline, and 500 mg/L glutamine could improve the callus induction rate and have a higher green seedling rate and a lower white seedling rate. Compared to HD9802S, two doubled haploid lines containing R6 with stable fertility showed significantly enhanced resistance to rice blast and no significant difference in spikelet number per panicle, 1000-grain weight, or grain shape. Our findings highlight a rapid and effective method for improving rice blast resistance in TGMS lines.
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