Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish
文献类型： 外文期刊
作者： Xu, Huan 1 ; Tong, Guangxiang 1 ; Yan, Ting 1 ; Dong, Le 1 ; Yang, Xiaoxing 1 ; Dou, Dongyu 1 ; Sun, Zhipeng 1 ; Liu, Tianqi 1 ; Zheng, Xianhu 1 ; Yang, Jian 6 ; Sun, Xiaowen 1 ; Zhou, Yi 7 ; Kuang, Youyi 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongliang River Fisheries Res Inst, Harbin, Peoples R China
2.Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Biotechnol & Breeding, Harbin, Peoples R China
4.Harbin Med Univ, Affiliated Hosp 2, Heilongliang Prov Key Lab Hard Tissue Dev & Regen, Harbin, Peoples R China
5.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
6.Zhejiang Wanli Univ, Inst Mariculture Breeding & Seed Ind, Ningbo, Peoples R China
7.Harvard Med Sch, Boston Childrens Hosp, Div Hematol Oncol, Stem Cell Program, Boston, MA 02115 USA
8.Harvard Med Sch, Dana Farber Canc Inst, Boston, MA 02115 USA
关键词： intermuscular bones; zebrafish; BMP6; bone development; SIK1
期刊名称：FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY （ 影响因子：6.081； 五年影响因子：6.576 ）
ISSN： 2296-634X
年卷期： 2022 年 10 卷
页码：
收录情况： SCI
摘要： Intermuscular bones (IBs) are small, hard-boned spicules located in the muscle tissue that mainly exist in the myosepta of lower teleosts, which hurt the edibleness and economic value of fish. The study of the development of lBs is very important for freshwater aquaculture fish, but the molecular mechanism of its formation and the key regulatory genes remain unclear. In this study, we first constructed two types of zebrafish mutants (the mutants losing lBs and the mutants with partial deletion of IBs) by knocking out bmp6. We then carried out a transcriptomic analysis to reveal the role of bmp6 in the developmental mechanism of IBs; we used the caudal musculoskeletal tissues of these mutants and wild-type zebrafish at three development stages (20, 45, and 60 dph) to perform transcriptomic analysis. The results showed that the deficiency of bmp6 upregulated sik1 and activated the TNF-A signaling via the NF-KB pathway, which inhibited the development of osteoblasts and promoted osteoclast formation, thereby inhibiting the formation of lBs. These results provided insights to understand the role of bmp6 in the development of lBs in zebrafish and are useful for selective breeding of lBs in cyprinids.
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