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Synthesis of flavour-related linalool is regulated by PpbHLH1 and associated with changes in DNA methylation during peach fruit ripening

文献类型： 外文期刊

作者： Wei, Chunyan 1 ; Liu, Hongru 1 ; Cao, Xiangmei 1 ; Zhang, Minglei 1 ; Li, Xian 1 ; Chen, Kunsong 1 ; Zhang, Bo 1 ;

作者机构： 1.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Lab Fruit Qual Biol, Hangzhou, Peoples R China

2.Shanghai Acad Agr Sci, Res Ctr Agr Prod Preservat & Proc, Shanghai, Peoples R China

关键词： fruit aroma; linalool; transcription factor; epigenetics

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2021 年 19 卷 10 期

页码：

收录情况： SCI

摘要： Linalool is one of the common flavour-related volatiles across the plant kingdom and plays an essential role in determining consumer liking of plant foods. Although great process has been made in identifying terpene synthase (TPS) genes associated with linalool synthesis, much less is known about regulation of this pathway. We initiated study by identifying PpTPS3 encoding protein catalysing enantiomer (S)-(+)-linalool synthesis, which is a major linalool component (similar to 70%) observed in ripe peach fruit. Overexpression of PpTPS3 led to linalool accumulation, while virus-induced gene silencing of PpTPS3 led to a 66.5% reduction in linalool content in peach fruit. We next identified transcription factor (TF) PpbHLH1 directly binds to E-box (CACATG) in the PpTPS3 promoter and activates its expression based on yeast one-hybrid assay and EMSA analysis. Significantly positive correlation was also observed between PpbHLH1 expression and linalool production across peach cultivars. Peach fruit accumulated more linalool after overexpressing PpbHLH1 in peach fruit and reduced approximately 54.4% linalool production after silencing this TF. DNA methylation analysis showed increased PpTPS3 expression was associated with decreased 5 mC level in its promoter during peach fruit ripening, but no reverse pattern was observed for PpbHLH1. Arabidopsis and tomato fruits transgenic for peach PpbHLH1 synthesize and accumulate higher levels of linalool compared with wild-type controls. Taken together, these results would greatly facilitate efforts to enhance linalool production and thus improve flavour of fruits.

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