您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a Monoclonal Antibody to Pig CD69 Reveals Early Activation of T Cells in Pig after PRRSV and ASFV Infection

文献类型： 外文期刊

作者： Tian, Yunfei 1 ; Hao, Yuxin 2 ; Dong, Maoli 1 ; Li, Shuai 1 ; Wang, Dongyue 2 ; Jiang, Fei 2 ; Wang, Qingqing 2 ; Hao, Xiaoli 1 ; Yang, Yi 1 ; Chen, Nanhua 1 ; Zhu, Jianzhong 1 ; Guo, Junqing 4 ; Wu, Jiajun 2 ; Shang, Shaobin 1 ; Zhou, Jiyong 6 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Inst Comparat Med, Yangzhou 225009, Jiangsu, Peoples R China

2.China Anim Dis Control Ctr, Biosafety High Level Lab Management Off, Beijing 102609, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

4.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450008, Peoples R China

5.Yangzhou Univ, Int Corp Lab Agr & Agr Prod Safety, Yangzhou 225009, Jiangsu, Peoples R China

6.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China

关键词： porcine CD69; monoclonal antibody; T cell activation; PRRSV; ASFV

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 6 期

页码：

收录情况： SCI

摘要： The CD69 molecule, as an early activation marker of lymphocytes, is often used to assess the activation of cellular immunity. However, for pigs, an anti-pig CD69 antibody is not yet available for this purpose after infection or vaccination. In this study, a monoclonal antibody (mAb) against pig CD69 was produced by peptide immunization and hybridoma technique. One mAb (5F12) showed good reactivity with pig CD69 that was expressed in transfected-HEK-293T cells and on mitogen-activated porcine peripheral blood mononuclear cells (PBMCs) by indirect immunofluorescence assay and flow cytometry. This mAb did not cross-react with activated lymphocytes from mouse, bovine, and chicken. Epitope mapping showed that the epitope recognized by this mAb was located at amino acid residues 147-161 of pig CD69. By conjugating with fluorochrome, this mAb was used to detect the early activation of lymphocytes in PRRSV- and ASFV-infected pigs by flow cytometry. The results showed that PRRSV infection induced the dominant activation of CD4 T cells in mediastinal lymph nodes and CD8 T cells in the spleen at 14 days post-infection, in terms of CD69 expression. In an experiment on ASFV infection, we found that ASFV infection resulted in the early activation of NK cells, B cells, and distinct T cell subsets with variable magnitude in PBMCs, spleen, and submandibular lymph nodes. Our study revealed an early event of lymphocyte and T cell activation after PRRSV and ASFV infections and provides an important immunological tool for the in-depth analysis of cellular immune response in pigs after infection or vaccination.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台