文献类型： 外文期刊

作者： Men, Yan ¹ ; Li, Ji-Rui ¹ ; Shen, Hai-Lin ² ; Yang, Yi-Ming ³ ; Fan, Shu-Tian ³ ; Li, Kun ¹ ; Guo, Yin-Shan ¹ ; Lin, Hong ¹ ; Liu, Zhen-Dong ¹ ; Guo, Xiu-Wu ¹ ;

作者机构： 1.Shenyang Agr Univ, Coll Hort, Shenyang, Peoples R China

2.Jilin Acad Agr Sci, Inst Pomol, Gongzhuling, Peoples R China

3.Chinese Acad Agr Sci, Inst Special Wild Econ Anim & Plant Sci, Changchun, Peoples R China

关键词： sex; VaAPRT3; marker; cytokinin; Vitis amurensis

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.772； 五年影响因子：4.933 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： In the past decade, progress has been made in sex determination mechanism in Vitis. However, genes responsible for sexual differentiation and its mechanism in V. amurensis remain unknown. Here, we identify a sex determination candidate gene coding adenine phosphoribosyl transferase 3 (VaAPRT3) in V. amurensis. Cloning and sequencing of the VaAPRT3 gene allowed us to develop a molecular marker able to discriminate female individuals from males or hermaphrodites based on a 22-bp InDel. Gene expression and endogenous cytokinin content analysis revealed that the VaAPRT3 gene is involved in sex determination or, to be precise, in female organ differentiation, through regulating cytokinin metabolism in V. amurensis. This study enlarged the understanding of sex determination mechanism in the genus Vitis, and the sex marker could be used as a helpful tool for sexual identification in breeding programs as well as in investigation and collection of V. amurensis germplasms.