文献类型： 外文期刊

作者： Qiao, Xinmeng ¹ ; Wang, Ruyuan ¹ ; Liu, Lanying ² ; Cui, Boya ¹ ; Zhao, Xinrui ¹ ; Yin, Min ¹ ; Li, Pirui ¹ ; Feng, Xu ¹ ; Shan, Yu ² ;

作者机构： 1.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Jiangsu Key Lab Res & Utilizat Plant Resources, Nanjing 210014, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Wolfberry Engn Technol, Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Peoples R China

关键词： salt stress; germination; photosynthesis; transcriptome; carbon fixation of Calvin cycle

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 15 期

页码：

收录情况： SCI

摘要： Since the onset of industrialization, the safety of arable land has become a pressing global concern, with soil salinization emerging as a critical threat to agricultural productivity and food security. To address this challenge, the cultivation of economically valuable salt-tolerant plants has been proposed as a viable strategy. In the study, we investigated the physiological and molecular responses of Lycium ruthenicum Murr. to varying NaCl concentrations. Results revealed a concentration-dependent dual effect: low NaCl levels significantly promoted seed germination, while high concentrations exerted strong inhibitory effects. To elucidate the mechanisms underlying these divergent responses, a combined analysis of metabolomics and transcriptomics was applied to identify key metabolic pathways and genes. Notably, salt stress enhanced photosynthetic efficiency through coordinated modulation of ribulose 5-phosphate and erythrose-4-phosphate levels, coupled with the upregulation of critical genes encoding RPIA (Ribose 5-phosphate isomerase A) and RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase). Under low salt stress, L. ruthenicum maintained intact cellular membrane structures and minimized oxidative damage, thereby supporting germination and early growth. In contrast, high salinity severely disrupted PS I (Photosynthesis system I) functionality, blocking energy flow into this pathway while simultaneously inducing membrane lipid peroxidation and triggering pronounced cellular degradation. This ultimately suppressed seed germination rates and impaired root elongation. These findings suggested a mechanistic framework for understanding L. ruthenicum adaptation under salt stress and pointed out a new way for breeding salt-tolerant crops and understanding the mechanism.