Isolation of a choline monooxygenase cDNA clone from Amaranthus tricolor and its expressions under stress conditions
文献类型： 外文期刊
作者： Meng, YL 1 ; Wang, YM 2 ; Zhang, DB 2 ; Nii, N 2 ;
作者机构： 1.Meijo Univ, Fac Agr, Tempaku Ku, Nagoya, Aichi 4688502, Japan
2.Meijo Univ, Fac Agr, Tempaku Ku, Nagoya, Aichi 4688502, Japan; Shaoguan Univ, Yingdong Coll Biotechnol, Shaoguan 512005, Peoples R China; Shanghai Acad Agr Sci, AgroBiotech Ctr, Shanghai Key Lab Genet Breeding, Shanghai 201106, Peoples R China
关键词： Amaranthus tricolor;betaine aldehyde dehydrogenase (BADH);choline monooxygenase (CMO);glycine betaine (GB);stress
期刊名称：CELL RESEARCH （ 影响因子：25.617； 五年影响因子：25.924 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Plants synthesize the osmoprotectant glycine betaine (GB) via choline->betaine aldehyde->glycine betaine[l]. Two enzymes are involved in the pathway, choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (l,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69 percent identity with CMOs in Spinacia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMOgene in the A. tricolor genome. The expressions of CMO and BADH proteins in A.tricolor leaves significantly increased under salinization, drought and heat stress (42 deg C), as determined by immunoblot analysis, but did not respond to cold stress (4 degC), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.
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