Single Multiple Cross Displacement Amplification for Rapid and Real-Time Detection of Porcine Circovirus 3
文献类型： 外文期刊
作者： Bian, Zhibiao 1 ; Cai, Rujian 1 ; Jiang, Zhiyong 1 ; Song, Shuai 1 ; Li, Yan 1 ; Chu, Pinpin 1 ; Zhang, Kunli 1 ; Yang, D 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China
2.Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou, Peoples R China
3.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming, Peoples R China
4.Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou, Peoples R China
关键词： PCV3; single multiple cross displacement amplification; detection; probe; quantitative
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）
ISSN：
年卷期： 2021 年 8 卷
页码：
收录情况： SCI
摘要： Since 2016, a novel porcine circovirus, PCV3, has been infecting pigs, causing significant economic losses to the pig industry. In recent years, the infection rate of PCV3 has been increasing, and thus rapid and accurate detection methods for PCV3 are essential. In this study, we established a novel probe-based single multiple cross displacement amplification (P-S-MCDA) method for PCV3. The method was termed as P-S-MCDA. The P-S-MCDA uses seven primers to amplify the capsid gene, and the assay can be performed at 60 degrees C for 30 min, greatly shortening the reaction time. The results of P-S-MCDA can not only be monitored in real time through fluorescence signals but also be determined by observing the fluorescence of the reaction tubes using a smartphone-based cassette. This method demonstrated good specificity and the same sensitivity as qPCR, with a minimum detection limit of 10 copies. In 139 clinical samples, the coincidence rate with qPCR was 100%. The P-S-MCDA can be widely applied in PCV3 detection in laboratories or in rural areas.
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