Rapid and specific detection of Chinook salmon bafinivirus (CSBV) in flatfish using loop-mediated isothermal amplification (LAMP)
文献类型： 外文期刊
作者： Su, Huaxing 1 ; Zhang, Yitong 2 ; He, Zhongwei 2 ; Yang, Yucong 2 ; Ren, Yuqin 2 ; Cao, Wei 2 ; Liu, Yufeng 2 ; Ren, Jiangong 2 ; Wang, Yufen 2 ; Wang, Guixing 2 ; Hou, Jilun 2 ;
作者机构： 1.Agr Univ Hebei, Ocean Coll, Qinhuangdao 066009, Peoples R China
2.Chinese Acad Fishery Sci, Hebei Key Lab Bohai Sea Fish Germplasm Resources C, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
3.Chinese Acad Fishery Sci, Bohai Sea Fishery Res Ctr, Qinhuangdao 066100, Peoples R China
关键词： Chinook salmon bafinivirus; LAMP; Flatfish disease; Rapid detection method
期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.9； 五年影响因子：2.6 ）
ISSN： 0967-6120
年卷期： 2023 年
页码：
收录情况： SCI
摘要： It is now understood that the flatfish farming industry faces significant threats from Chinook salmon bafinivirus (CSBV) induced hemorrhagic disease. Developing a prompt and efficient diagnostic method is crucial for effective disease control and minimizing losses in aquaculture production. This research assessed the loop-mediated isothermal amplification (LAMP) technique, using a specific primer set, for rapid and precise detection of CSBV. The primer set for LAMP detection was selected based on its demonstrated high specificity and sensitivity, as confirmed through visualization and gel electrophoresis. The CSBV-LAMP reaction system operated at an effective temperature of 63-67 & DEG;C for 60 min. Importantly, the LAMP assay could identify viral genomic cDNA at an extremely low concentration of 10 pg/& mu;L within 60 min at 65 & DEG;C. Moreover, this reaction system accurately detected CSBV in naturally infected flatfish samples, yielding a 100% positive detection rate.
- 相关文献
作者其他论文 更多>>
-
Functional analysis of the ube3a response in Japanese flounder (Paralichthys olivaceus) to CSBV infection
作者：Su, Huaxing;Zhang, Yitong;He, Zhongwei;Yang, Yucong;Ren, Yuqin;Cao, Wei;Liu, Yufeng;Ren, Jiangong;Wang, Yufen;Wang, Guixing;Hou, Jilun;Su, Huaxing;Gong, Chunguang;Zhang, Yitong;He, Zhongwei;Yang, Yucong;Ren, Yuqin;Cao, Wei;Liu, Yufeng;Ren, Jiangong;Wang, Yufen;Wang, Guixing;Hou, Jilun
关键词：Japanese flounder; ube3a; Ubiquitination; Inflammatory response
-
Establishment of recombinant doubled haploid lines of Japanese flounder and evaluation of their reproductive capacity
作者：Zhang, Xiaoyan;Wang, Guixing;San, Lize;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Wang, Yufen;Hou, Jilun;Zhang, Xiaoyan;Wang, Guixing;San, Lize;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Wang, Yufen;Hou, Jilun
关键词：Recombinant doubled haploid; Japanese flounder; Reproductive traits; Gynogenesis; Targeted breeding
-
The first case of gynogenesis induced by cold- and heat-shock treatment in Thamnaconus modestus
作者：Zhang, Xiaoyan;San, Lize;Yang, Yucong;Tao, Yuehong;Ren, Jiangong;Liu, Yufeng;He, Zhongwei;Di, Jiashuo;Pei, Ziteng;Wang, Guixing;Hou, Jilun;Zhang, Xiaoyan;San, Lize;Yang, Yucong;Tao, Yuehong;Ren, Jiangong;Liu, Yufeng;He, Zhongwei;Di, Jiashuo;Pei, Ziteng;Wang, Guixing;Hou, Jilun;Di, Jiashuo;Pei, Ziteng
关键词：Thamnaconus modestus; Meiotic gynogenesis; Molecular markers; Homogeneity
-
Role of Thyroid Hormone in Dynamic Variation of gdf6a Gene during Metamorphosis of Paralichthys olivaceus
作者：Shi, Yaxin;Qiu, Junqiang;Li, Xike;Lin, Yue;Li, Wenjuan;Fu, Yuanshuai;Shi, Yaxin;Qiu, Junqiang;Li, Xike;Lin, Yue;Li, Wenjuan;Fu, Yuanshuai;Hou, Jilun
关键词：Paralichthys olivaceus; gdf6a; thyroid hormone; thyroid hormone receptor; metamorphosis
-
Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection
作者：Sun, Zhaodi;Yang, Yucong;Fu, Yuanshuai;Sun, Zhaodi;Yang, Yucong;Fu, Yuanshuai;Sun, Zhaodi;Yang, Yucong;Fu, Yuanshuai;Sun, Zhaodi;Ren, Yuqin;Zhang, Yitong;Yang, Yucong;Wang, Guixing;He, Zhongwei;Liu, Yufeng;Cao, Wei;Wang, Yufen;Hou, Jilun
关键词：Paralichthys olivaceus; Cell line; Bohle virus; Chinese giant salamander iridovirus
-
Thyroid Hormone Signaling Is Required for Dynamic Variation in Opsins in the Retina during Metamorphosis of the Japanese Flounder (Paralichthys olivaceus)
作者：Shi, Yaxin;Ji, Wenyao;Li, Xike;Shi, Zhiyi;Li, Wenjuan;Fu, Yuanshuai;Shi, Yaxin;Ji, Wenyao;Li, Xike;Shi, Zhiyi;Li, Wenjuan;Fu, Yuanshuai;Shi, Yaxin;Ji, Wenyao;Li, Xike;Shi, Zhiyi;Li, Wenjuan;Fu, Yuanshuai;Shi, Yang;Hou, Jilun
关键词：Paralichthys olivaceus; opsin; thyroid hormone; metamorphosis
-
Population genetics analysis of the black rockfish Sebastes schlegelii in Northern China based on 2b-RAD simplified genome sequencing
作者：Cao, Wei;Zhang, Mingyi;Wu, Nan;Han, Haiying;Zhong, Rujie;Yu, Tao;Ren, Liqun;Zhao, Chunnuan;Li, Bo;Zheng, Yanxin;Wu, Nan;Zhong, Rujie;Wang, Xiaomei
关键词：
