Rapid and specific detection of Chinook salmon bafinivirus (CSBV) in flatfish using loop-mediated isothermal amplification (LAMP)
文献类型： 外文期刊
作者： Su, Huaxing 1 ; Zhang, Yitong 2 ; He, Zhongwei 2 ; Yang, Yucong 2 ; Ren, Yuqin 2 ; Cao, Wei 2 ; Liu, Yufeng 2 ; Ren, Jiangong 2 ; Wang, Yufen 2 ; Wang, Guixing 2 ; Hou, Jilun 2 ;
作者机构： 1.Agr Univ Hebei, Ocean Coll, Qinhuangdao 066009, Peoples R China
2.Chinese Acad Fishery Sci, Hebei Key Lab Bohai Sea Fish Germplasm Resources C, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
3.Chinese Acad Fishery Sci, Bohai Sea Fishery Res Ctr, Qinhuangdao 066100, Peoples R China
关键词： Chinook salmon bafinivirus; LAMP; Flatfish disease; Rapid detection method
期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.9； 五年影响因子：2.6 ）
ISSN： 0967-6120
年卷期： 2023 年
页码：
收录情况： SCI
摘要： It is now understood that the flatfish farming industry faces significant threats from Chinook salmon bafinivirus (CSBV) induced hemorrhagic disease. Developing a prompt and efficient diagnostic method is crucial for effective disease control and minimizing losses in aquaculture production. This research assessed the loop-mediated isothermal amplification (LAMP) technique, using a specific primer set, for rapid and precise detection of CSBV. The primer set for LAMP detection was selected based on its demonstrated high specificity and sensitivity, as confirmed through visualization and gel electrophoresis. The CSBV-LAMP reaction system operated at an effective temperature of 63-67 & DEG;C for 60 min. Importantly, the LAMP assay could identify viral genomic cDNA at an extremely low concentration of 10 pg/& mu;L within 60 min at 65 & DEG;C. Moreover, this reaction system accurately detected CSBV in naturally infected flatfish samples, yielding a 100% positive detection rate.
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