文献类型： 外文期刊
作者： Li, Mengling 1 ; Guo, Pengju 1 ; Chen, Cen 1 ; Feng, Helong 3 ; Zhang, Wanpo 3 ; Gu, Changqin 3 ; Wen, Guoyuan 5 ; Rao, V 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Vet Med, Key Lab Dev Vet Diagnost Prod, Minist Agr, Wuhan, Peoples R China
2.Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Peoples R China
3.Huazhong Agr Univ, Coll Vet Med, Div Pathol, Wuhan, Peoples R China
4.Hongshan Lab, Wuhan, Peoples R China
5.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan, Peoples R China
6.Catholic Univ Amer, Bacteriophage Med Res Ctr, Dept Biol, Washington, DC 20064 USA
关键词： flu vaccine; virus-like particle; bacteriophage T4 platform; extracellular domain of matrix protein 2; phage display
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.561； 五年影响因子：7.624 ）
ISSN： 1664-3224
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Developing influenza vaccines that protect against a broad range of viruses is a global health priority. Several conserved viral proteins or domains have been identified as promising targets for such vaccine development. However, none of the targets is sufficiently immunogenic to elicit complete protection, and vaccine platforms that can enhance immunogenicity and deliver multiple antigens are desperately needed. Here, we report proof-of-concept studies for the development of next-generation influenza vaccines using the bacteriophage T4 virus-like particle (VLP) platform. Using the extracellular domain of influenza matrix protein 2 (M2e) as a readout, we demonstrate that up to ~1,281 M2e molecules can be assembled on a 120 x 86 nanometer phage capsid to generate M2e-T4 VLPs. These M2e-decorated nanoparticles, without any adjuvant, are highly immunogenic, stimulate robust humoral as well as cellular immune responses, and conferred complete protection against lethal influenza virus challenge. Potentially, additional conserved antigens could be incorporated into the M2e-T4 VLPs and mass-produced in E. coli in a short amount of time to deal with an emerging influenza pandemic.
- 相关文献
作者其他论文 更多>>
-
Spray vaccination with a Newcastle disease virus (NDV)-vectored infectious laryngotracheitis (ILT) vaccine protects commercial chickens from ILT in the presence of maternally-derived antibodies
作者：Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Luo, Qingping;Wen, Guoyuan
关键词：ILTV; NDV; vector vaccine; spray vaccination; maternally derived antibodies; protection
-
The impacts of animal agriculture on One Health-Bacterial zoonosis, antimicrobial resistance, and beyond
作者：Zhang, Tengfei;Zhang, Wenting;Shang, Yu;Luo, Qingping;Wen, Guoyuan;Nickerson, Rhea;Cheng, Zhenyu;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Luo, Qingping
关键词：Zoonosis; Antimicrobial resistance
-
BacScan: a novel genome-wide strategy for uncovering broadly immunogenic proteins in bacteria
作者：Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Qin, Huan;Wen, Guoyuan
关键词：bacterial subunit vaccine; vaccine target; immunogenic protein; protective antigen; genome-wide screening; streptococcus suis
-
Porcine kobuvirus enhances porcine epidemic diarrhea virus pathogenicity and alters the number of intestinal lymphocytes in piglets
作者：Wu, Simin;Gou, Fang;Meng, Jie;Liu, Wanchen;Ding, Weishuai;Xu, Weihang;Gu, Changqin;Hu, Xueying;Cheng, Guofu;Tao, Pan;Zhang, Wanpo;Jin, Xin
关键词：Porcine kobuvirus; Porcine epidemic diarrhea virus; Co-infection; Pathogenicity; Intestinal lymphocytes
-
T4 bacteriophage nanoparticles engineered through CRISPR provide a versatile platform for rapid development of flu mucosal vaccines
作者：Li, Mengling;Chen, Cen;Wang, Xialin;Guo, Pengju;Feng, Helong;Zhang, Xueqi;Zhang, Wanpo;Gu, Changqin;Peng, Guiqing;Tao, Pan;Li, Mengling;Chen, Cen;Wang, Xialin;Guo, Pengju;Zhang, Xueqi;Peng, Guiqing;Tao, Pan;Feng, Helong;Wen, Guoyuan;Zhu, Jingen;Rao, Venigalla B.;Feng, Yaoyu;Xiao, Lihua
关键词：Mucosal vaccine; Influenza virus; Bacteriophage T4; Mucosal immune responses; CRISPR engineering
-
Pathological observation and transcriptomic analysis of thymus injury in PRRSV-infected piglets
作者：Su, Naying;Lin, Zhengdan;Liu, Xi;Sun, Xiuxiu;Jin, Xinxin;Feng, Helong;Zhan, Cunlin;Hu, Xueying;Gu, Changqin;Zhang, Wanpo;Cheng, Guofu;Su, Naying;Feng, Helong
关键词：Porcine reproductive and respiratory syndrome virus; Piglets; Thymus; Transcriptome; Histopathological findings
-
C-terminal truncation of the hemagglutinin-neuraminidase (HN) protein enhances the virulence and immunogenicity of Newcastle disease virus (NDV) vaccine strain V4
作者：Ren, Xiangfei;Zeng, Zhe;Shang, Yu;Yao, Lun;Li, Li;Zhang, Wenting;Guo, Yunqing;Wang, Hongcai;Zhang, Rongrong;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Ren, Xiangfei;Zeng, Zhe;Shang, Yu;Yao, Lun;Li, Li;Zhang, Wenting;Guo, Yunqing;Wang, Hongcai;Zhang, Rongrong;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Ren, Xiangfei;Hu, Sishun;Luo, Qingping
关键词：Newcastle disease virus; HN protein; C-terminal; Virulence; Immunogenicity
