您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transcriptome sequencing revealed the regulation of stem internode length associated with mechanical harvesting in three tea varieties

文献类型： 外文期刊

作者： Mi, Xiaozeng 1 ; Qiao, Dahe 1 ; Yang, Chun 1 ; Chen, Juan 1 ; Liang, Sihui 1 ; Guo, Yan 1 ;

作者机构： 1.Tea Res Inst, Guizhou Acad Agr Sci, Guiyang, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Key Lab Mol Breeding Characterist Hort Cro, Guiyang, Guizhou, Peoples R China

3.Guizhou Acad Agr Sci, Key Lab Crop Genet Resources & Germplasm Innovat K, Minist Agr & Rural Affairs, Guiyang 550006, Guizhou, Peoples R China

关键词： stem internode length; mechanical harvesting; tea plant; RNA-Seq; WGCNA

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Introduction Internode length of tea plant is one of the key traits affecting the mechanical harvesting effect, but there are relatively few reports on their molecular regulatory.Methods Transcriptome sequencing (RNA-seq) was performed on it in order to explain its molecular mechanism. GO, KEGG enrichment and WGCNA were used to identify key genes, and their expression levels in three tea tree varieties were validated by qRT-PCR.Result In this study, we measured the internode lengths of the three varieties and found that their internode lengths were 'Feiyun' > 'Qiancha 1' > 'Longjing 43'. 10,518 differentially expressed genes were identified through transcriptome sequencing and analysis. GO and KEGG enrichment showed that these differentially expressed genes were mainly enriched in plant hormone signal transduction and DNA-binding transcription factor activity pathways. WGCNA analysis identified two modules significantly correlated with internode length. Combining enrichment analysis with WGCNA results, 28 candidate genes associated with internode length were identified. In addition, it was found that the expression levels of DELLA and GA3ox were highly expressed in 'Longjing 43', while GA2ox, WRKY, and ERF were highly expressed in 'Feiyun', showing significant positive and negative correlations with internode length, respectively.Discussion Our results provide candidate genes for studying the molecular mechanism of stem elongation, and provide a theoretical basis for selection machine harvested tea varieties and improvement of mechanical harvesting efficiency.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台