A defensive pathway from NAC and TCP transcription factors activates a BAHD acyltransferase for (Z)-3-hexenyl acetate biosynthesis to resist herbivore in tea plant (Camellia sinensis)
文献类型： 外文期刊
作者： Gu, Honglian 1 ; Li, Jiaxing 1 ; Qiao, Dahe 1 ; Li, Mei 1 ; Yao, Yingjie 1 ; Xie, Hui 1 ; Huang, Ke-lin 1 ; Liu, Shengrui 1 ; Xie, De-Yu 3 ; Wei, Chaoling 1 ; Zhu, Junyan 1 ;
作者机构： 1.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Key Lab Tea Biol & Tea Proc, Minist Agr,Anhui Prov Lab Tea Plant Biol & Utiliz, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China
2.Guizhou Acad Agr Sci, Tea Res Inst, Minist Agr & Rural Affairs, Key Lab Crop Gene Resources & Germplasm Innovat Ka, Guiyang 550006, Guizhou, Peoples R China
3.North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA
关键词：
(Z)-3-hexenyl acetate;
期刊名称：NEW PHYTOLOGIST （ 影响因子：8.1； 五年影响因子：10.3 ）
ISSN： 0028-646X
年卷期： 2025 年 245 卷 3 期
页码：
收录情况： SCI
摘要： Numerous herbivore-induced plant volatiles (HIPVs) play important roles in plant defense. In tea plants (Camellia sinensis), (Z)-3-hexenyl acetate (3-HAC) has been characterized as associated with resistance to herbivores. To date, how tea plants biosynthesize and regulate 3-HAC to resist herbivores remain unclear. Based on transcriptomes assembled from Ectropis obliqua-fed leaves, a cDNA encoding BAHD acyltransferase, namely CsCHAT1, was highly induced in leaves fed with E. obliqua. Enzymatic assays showed that CsCHAT1 converted (Z)-3-hexenol into 3-HAC. Further suppression of CsCHAT1 expression reduced the accumulation of 3-HAC and lowered the resistance of tea plants to E. obliqua, while 3-HAC replenishment rescued the reduced resistance of CsCHAT1-silenced tea plants against E. obliqua. Two transcription factors (TFs), CsNAC30 and CsTCP11, were co-expressed with CsCHAT1. An integrative approach of biochemistry, DNA-protein interaction, gene silencing, and metabolic profiling revealed that the two TFs positively regulated the expression of CsCHAT1. The suppression of either one decreased the production of 3-HAC and eliminated the resistance of tea plants to E. obliqua. Notably, the suppression of either one considerably impaired JA-induced 3-HAC biosynthesis in tea plant. The proposed pathway can be targeted for innovative agro-biotechnologies protecting tea plants from damage by E. obliqua.
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