您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Next-Generation DNA Barcoding for Fish Identification Using High-Throughput Sequencing in Tai Lake, China

文献类型： 外文期刊

作者： Mu, Yawen 1 ; Song, Chao 3 ; Yang, Jianghua 1 ; Zhang, Yong 2 ; Zhang, Xiaowei 1 ;

作者机构： 1.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource, Nanjing Univ Xianlin Campus,163 Xianlin Ave, Nanjing 210023, Peoples R China

2.Jiangsu Prov Environm Monitoring Ctr, Nanjing 210019, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China

关键词： high-throughput sequencing; DNA barcoding; cytochrome c oxidase I; fish species identification; Tai Lake; metabarcoding

期刊名称：WATER （ 影响因子：3.4； 五年影响因子：3.5 ）

ISSN：

年卷期： 2023 年 15 卷 4 期

页码：

收录情况： SCI

摘要： Tai Lake, an important biodiversity hotspot of the lower reaches of the Yangtze River in China, possesses its characteristic fish fauna. Barcoding on native species is important for species identification and biodiversity assessment with molecular-based methods, such as environmental DNA (eDNA) metabarcoding. Here, DNA-barcoding coupled with high-throughput sequencing (HTS) and traditional Sanger sequencing was introduced to barcoding 180 specimens belonging to 33 prior morphological species, including the most majority of fish fauna in Tai Lake. HTS technology, on the one hand, significantly enhances the capture of barcode sequences of fish. The successful rate of fish barcoding was 74% and 91% in Sanger and HTS, respectively. On the other hand, the HTS output has a large number (64%) of insertions and deletions, which require strict bioinformatics processing to ensure that the ''true'' barcode sequence is captured. Cross-contamination and parasites were the primary error sources that compromised attempts at the DNA barcoding of fish species. The barcode gap analysis was 100% successful at delimiting species in all specimens. The automatic barcode gap discovery (ABGD) method grouped barcode sequences into 34 OTUs, and some deep divergence and closed species failed to obtain corresponding OTUs. Overall, the local species barcode library established by HTS barcoding here is anticipated to shed new light on conserving fish diversity in Tai Lake.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台