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Characterization of a novel shrimp pathogen, Vibrio brasiliensis, isolated from Pacific white shrimp, Penaeus vannamei

文献类型： 外文期刊

作者： Li, Ge 1 ; Xie, Guosi 2 ; Wang, Hailiang 3 ; Wan, Xiaoyuan 2 ; Li, Xinshu 1 ; Shi, Chengyin 2 ; Wang, Ziyan 2 ; Gong, Mia 1 ;

作者机构： 1.Jiangsu Ocean Univ, Sch Marine Sci & Fisheries, Lianyungang, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Marine Aquaculture Epidemiol & Biosecur, Key Lab Marine Aquaculture Dis Control,Minist Agr, 106 Nanjing Rd, Qingdao 266071, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

4.Hainan Zhongzheng Aquat Sci & Technol Co Ltd, Dongfang, Peoples R China

5.Network Aquaculture Ctr Asia Pacific, Bangkok, Thailand

关键词： black gill disease; pathogenicity; Penaeus vannamei; Vibrio brasiliensis

期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）

ISSN： 0140-7775

年卷期： 2021 年 44 卷 10 期

页码：

收录情况： SCI

摘要： A novel pathogenic strain Vibrio 20190611023 was isolated from the hepatopancreas of moribund cultured Penaeus vannamei suffering from black gill disease. This strain was identified as V. brasiliensis based on the phylogenetic analyses of 16S rDNA gene and five other housekeeping genes (i.e., gapA, ftsZ, mreB, topA and gyrB). Some biochemical features of this strain were determined with an API 20NE system, and its haemolytic activity was determined using a sheep blood agar plate. The pathogenicity of this isolate 20190611023 was confirmed by the experimental challenge tests and histopathological examinations. P. vannamei were challenged via reverse gavage with different doses of bacterial suspensions. The calculated median lethal dose (LD50) was (3.16 +/- 1.78) x 10(5) CFU/g (body weight). Moreover, antibiotic susceptibility tests were performed, the results of which showed that the strain 20190611023 was sensitive to chloramphenicol, compound sulphamethoxazole, ciprofloxacin, doxycycline and oxacillin, but resistant to erythromycin, kanamycin, gentamicin, cefoperazone, ceftriaxone, cefamezin and piperacillin. To our knowledge, this is the first report for demonstrating V. brasiliensis as a shrimp pathogen, which expands the host range of V. brasiliensis infection. The present study highlights that more attention should be paid to this novel pathogen in intensive shrimp aquaculture.

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