Type II interferons (IFN-γ and IFN-γrel) activate downstream genes through various potential receptor combinations to exert antiviral functions in spotted sea bass (Lateolabrax maculatus)
文献类型： 外文期刊
作者： Yan, Lulu 1 ; Guo, Jieyun 1 ; Zhao, Chao 1 ; Wang, Pengfei 1 ; Zhang, Bo 1 ; Qiu, Lihua 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou, Peoples R China
2.Sanya Trop Fisheries Res Inst, Sanya, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Aquat Genom, Minist Agr & Rural Affairs, Beijing, Peoples R China
关键词： Lateolabrax maculatus; Type II IFN; CRFB; ISGs; LBUSV
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2024 年 145 卷
页码：
收录情况： SCI
摘要： Type II interferons (IFNs) exert antiviral functions by binding to receptors and activating downstream signaling pathways. However, our understanding of the antiviral functions and the receptor complex model of type II IFNs in teleost fish remains limited. In this study, we determined the functions of type II IFNs (LmIFN-gamma and LmIFN gamma rel) in Lateolabrax maculatus and assessed their antiviral ability mediated by their combination with different cytokine receptor family B members (LmCRFB6, LmCRFB13, and LmCRFB17). After infection with largemouth bass ulcer syndrome virus (LBUSV), the expression levels of LmIFNs and LmCRFBs increased significantly in vitro and in vivo. Incubation or injection with LmIFNs-His activated the expressions of LmISG15, LmMx, and LmIRF1. LmIFN-gamma and LmIFN-gamma rel both bound to the extracellular domains of the three CRFBs via Pull-down. Furthermore, LmIFN-gamma combined with LmCRFB6, LmCRFB6+LmCRFB13, and LmCRFB6+LmCRFB13+LmCRFB17 and LmIFN-gamma rel combined with all combinations containing LmCRFB17 induced the transcription of downstream genes and reduced the number of LBUSV copies. Therefore, type II IFNs (LmIFN-gamma and LmIFN-gamma rel) contribute to enhanced antiviral immunity in L. maculatus and that ligand-receptor combinations effectively suppress virus replication. These findings provide a reference for future studies of the signal transduction mechanism of type II IFNs in teleost fish.
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