Characterization and immune role of class B scavenger receptor member 1 in spotted sea bass (Lateolabrax maculatus)
文献类型： 外文期刊
作者： Fu, Yichen 1 ; Zhao, Chao 2 ; Lin, Changhong 1 ; Zhang, Bo 2 ; Yan, Lulu 2 ; Wang, Pengfei 2 ; Qiu, Lihua 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou, Peoples R China
3.Sanya Trop Fisheries Res Inst, Sanya, Peoples R China
4.Chinese Acad Fishery Sci, Key Lab Aquat Genom, Minist Agr & Rural Affairs, Beijing, Peoples R China
关键词： Lateolabrax maculatus; SRB1; Aeromonas veronii; Immune response
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）
ISSN： 1050-4648
年卷期： 2024 年 153 卷
页码：
收录情况： SCI
摘要： Scavenger receptors (SRs) are integral to the innate immune system and function as pattern-recognition receptors that facilitate pathogen clearance and mediate anti-inflammatory responses. However, the role of SRs in the immune response of Lateolabrax maculatus against Aeromonas veronii is unclear. Here, we cloned scavenger receptor B1 from L. maculatus (LmSRB1) and performed bioinformatics analysis to study its potential functions. The open reading frame spans 1530 base pairs and encodes a 509-amino acid protein with a molecular mass of 57.44 kDa. Comparative analysis revealed high sequence conservation among fish species. Expression profiling revealed strong LmSRB1 transcription in various tissues, especially in head kidney and spleen. Following A. veronii exposure, LmSRB1 expression initially increased, peaking after 4-8 h, with a notable secondary peak at 72 h. Fluorescence in situ hybridization indicated that LmSRB1 mainly localized to the cytoplasm, and subcellularlocalization studies confirmed LmSRB1 protein expression in the cytoplasm and cell membrane. Enzyme-linked immunosorbent assay data showed dose-dependent binding of LmSRB1 to A. veronii. Modulating LmSRB1 expression significantly altered the levels of IL-8, IL-1 beta, TRAF6, and NIK. These results highlight the crucial role of LmSRB1 in L. maculatus's innate immune response to A. veronii and offer insights into improving the management of bacterial infections in aquaculture.
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