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Overexpression of a beta-1,6-glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

文献类型： 外文期刊

作者： Shen, Enlong 1 ; Wang, Xingchao 1 ; Lu, Zhaoxi 1 ; Zhou, Fei 1 ; Ma, Weihua 1 ; Cui, Zhongli 1 ; Li, Zhoukun 1 ; Li, Changyan 1 ; Lin, Yongjun 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Life Sci & Technol, Natl Ctr Plant Gene Res, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr & Rural Affairs, Nanjing, Peoples R China

3.Nanjing Agr Univ, Key Lab Biol Interact & Crop Hlth, Nanjing, Peoples R China

4.Food Crops Inst, Hubei Acad Agr Sci, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Wuhan, Peoples R China

关键词： glucan; beta-1; 6-glucanase gene; GluM; rice blast; sheath blight; rice false smut

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2023 年 79 卷 6 期

页码：

收录情况： SCI

摘要： BACKGROUND: Frequent fungal diseases tend to lead to severe losses in rice production. As a main component of the fungal cell wall, glucan plays an important role in the growth and development of fungi. Glucanase can inhibit the growth of fungi by breaking glycosidic bonds, and may be a promising target for developing rice varieties with broad-spectrum disease resistance.RESULTS: We transferred a codon-optimized beta-1,6-glucanase gene (GluM) from myxobacteria into the japonica rice variety Zhonghua11 (ZH11), and obtained a large number of individual transgenic plants with GluM overexpression. Based on molecular analysis, three single-copy homozygous lines with GluM overexpression were selected for assessment of fungal disease resistance at the T-3 generation. Compared with that of the recipient cultivar ZH11, the area of rice blast lesion in transgenic rice was reduced by 82.71%; that of sheath blight lesion was decreased by 35.76%-43.67%; the sheath blight resistance in the field was enhanced by an average of 0.75 grade over 3 years; and the incidence of diseased panicles due to rice false smut was decreased by 65.79%. More importantly, there was no obvious loss of yield (without a significant effect on agronomic traits). Furthermore, plants overexpressing a beta-1,6-glucanase gene showed higher disease resistance than rice plants overexpressing a beta-1,3-glucanase gene derived from tobacco.CONCLUSION: The beta-1,6-glucanase gene GluM can confer broad-spectrum disease resistance to rice, providing an environmentally friendly alternative way to effectively manage fungal pathogens in rice production. (c) 2023 Society of Chemical Industry.

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