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Cucumber abscisic acid 8 & PRIME;-hydroxylase Csyf2 regulates yellow flesh by modulating carotenoid biosynthesis

文献类型： 外文期刊

作者： Wang, Xueting 1 ; Jin, Boyan 1 ; Yan, Wenjing 1 ; Wang, Jiaxi 1 ; Xu, Jun 1 ; Cai, Congxi 1 ; Qi, Xiaohua 1 ; Xu, Qiang 1 ; Yang, Xiaodong 1 ; Xu, Xuewen 1 ; Chen, Xuehao 1 ;

作者机构： 1.Yangzhou Univ, Sch Hort & Landscape Architecture, Dept Hort, Yangzhou 225009, Peoples R China

2.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou 225009, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Cucumber (Cucumis sativus L.) flesh is typically colorless or pale green. Flesh with yellow or orange pigment, determined mainly by carotenoid content and composition, is mostly found in semi-wild Xishuangbanna cucumber, which has a very narrow genetic background. Here, we identified a spontaneous cucumber mutant with yellow flesh (yf-343), which accumulated more & beta;-cryptoxanthin and less lutein than regular cultivated European glasshouse-type cucumbers. Genetic analysis revealed that the yellow flesh phenotype was controlled by a single recessive gene. Through fine mapping and gene sequencing, we identified the candidate gene C. sativus yellow flesh 2 (Csyf2), encoding an abscisic acid (ABA) 8 & PRIME;-hydroxylase. Overexpression and RNAi-silencing of Csyf2 in cucumber hairy roots produced lower and higher ABA contents than in non-transgenic controls, respectively. Further, RNA-seq analysis suggested that genes related to ABA signal transduction were differentially expressed in fruit flesh between yf-343 and its wild type, BY, with white flesh. The carotenoid biosynthesis pathway was specifically enriched in fruit flesh at 30 days after pollination when yf-343 fruit flesh turns yellow. Our findings highlight a promising target for gene editing to increase carotenoid content, expanding our genetic resources for pigmented cucumber flesh breeding for improving the nutritional quality of cucumber. Abscisic acid 8 & PRIME;-hydroxylase, a key enzyme modulating endogenous abscisic acid homeostasis, adjusts carotenoid composition to produce yellow flesh in cucumber (Cucumis sativus L.).

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