文献类型： 外文期刊
作者： Zhu, Fuquan 1 ; Yang, Meng 1 ; Wang, Dayu 1 ; Jiang, Yuan 1 ; Jia, Chao 1 ; Fu, Yanfeng 2 ; Yu, Aochen 1 ; Liu, Huijun 3 ; Wang, Meixia 3 ; Wang, Tingzhang 3 ; Liu, Honglin 1 ; Li, Juan 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing Weigang 1, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China
3.Zhejiang Inst Microbiol, Key Lab Microbial Technol & Bioinformat Zhejiang P, Hangzhou, Zhejiang, Peoples R China
关键词： Porcine; transcriptomics; proteomics; maternal factors; oriented bisection
期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）
ISSN： 1049-5398
年卷期： 2024 年 35 卷 1 期
页码：
收录情况： SCI
摘要： It is known that asymmetrical maternal transcripts play an important role in the cell fate of the early embryo, but few studies are available in mammal oocytes especially in pig. To investigate the spatial factors in pig oocytes, the oriented bisection was established for collecting karyoplasts (NSOs) and cytoplasts (SSOs) with more than 95% efficiency. Subsequently, RNA-Seq and LC-MS/MS analysis were performed on NSOs and SSOs. Although no differentially expressed genes (DEGs) could be detected between NSOs and SSOs, 89 of the differentially expressed proteins (DEPs) were detected, that 58 proteins higher expressed but 31 proteins lower expressed in NSOs compared with SSOs. These DEPs mainly participated in the 'cell cycle' and 'ribosome' pathway, while the up-regulated DEPs were mainly GO in 'spindle' and 'positive regulation of translation', and the down-regulated DEPs were in 'cytosolic small ribosomal subunit' and 'mRNA binding'. The up-regulated DEP SIRT5 which are related to the regulation of gene expression, epigenetic were further detected and revealed. A spatial asymmetry of maternal factors at the protein level was firstly detected in pig mature oocytes.
- 相关文献
作者其他论文 更多>>
-
Kynurenine, a derivative of tryptophan, inhibits progesterone biosynthesis in porcine granulosa luteal cells through Aryl hydrocarbon receptor-mediated downregulation of GATA-binding protein 4, 6, and CCAAT/enhancer-binding protein beta†
作者：Liao, Shiying;Deng, Yanfei;Liao, Shiying;Cheng, Jinhua;Zhao, Weimin;Dai, Chaohui;Fu, Yanfeng;Li, Bixia;Li, Hui;Li, Hui
关键词：kynurenine; progesterone; granulosa luteal cell; STAR; AHR
-
Recombinant canine distemper virus expressing virus-like particle VP2 protein of mink enteritis virus protects minks against lethal challenges of both viruses
作者：Jiang, Yuan;Sun, Yiyang;Zhai, Boyu;Chen, Tianjie;Li, Yang;Ren, Lina;Ma, Yuchen;Han, Kun;Zhao, Jianjun;Wang, Jianke;Bi, Zhenwei;Hu, Bo
关键词：Mink; Canine distemper virus; Mink enteritis virus; Capsid protein 2; Virus-like particles; Bivalent live vaccine
-
The Erk1/2-EGR1 signaling pathway is involved in lipopolysaccharide-induced transforming growth factor-beta 1 expression in mouse macrophages
作者：Zhao, Weimin;Wang, Hong;Dai, Chaohui;Li, Bixia;Fu, Yanfeng;Cheng, Jinhua;Li, Hui;Wang, Hong;Li, Hui
关键词：LPS; TGF-beta 1; Erk1/2; EGR1; Macrophage
-
Chlorogenic acid alleviates deoxynivalenol-induced damage in porcine trophectoderm cells by regulating the PI3K/AKT signaling pathway†
作者：Dai, Chaohui;Cheng, Jinhua;Li, Hui;Zhao, Weimin;Li, Bixia;Fu, Yanfeng;Wang, Xuemin;Liao, Chao;Yan, Junshu;Dai, Chaohui;Cheng, Jinhua;Li, Hui;Zhao, Weimin;Li, Bixia;Fu, Yanfeng;Wang, Xuemin;Liao, Chao;Yan, Junshu;Chen, Yanyu
关键词：pig; placental trophoblast cells; deoxynivalenol; chlorogenic acid; cell damage
-
Current trends and possibilities of typical microbial protein production approaches: a review
作者：He, JinTao;Tang, Min;Zhong, FeiFei;Deng, Jing;Li, Wen;Zhang, Lin;Lin, QinLu;Li, Juan;Zhong, FeiFei;Li, Wen;Lin, QinLu;Li, Wen;Lin, QinLu;Xia, Xu;Guo, Ting
关键词：Microbial protein; synthesis process; organic waste; one-carbon compounds; resource valorization; application
-
6-Gingerol and Astaxanthin Mitigate the Effects of Stearic Acid in Pig Oocyte Maturation
作者：Saeed, Hussain Ahmad;Lu, Xinyue;Jiang, Yuan;Koutonin, Bienvenu Odjoubire Mahougnon;Wang, Dayu;Jia, Chao;Li, Juan;Sabir, Rabia;Fu, Yangfeng
关键词：6-Gingerol; Astaxanthin; in vitro maturation; oocytes; pig; stearic acid
-
Long-term organic fertilization enhances potassium uptake and yield of sweet potato by expanding soil aggregates-associated potassium stocks
作者：Yuan, Jie;Ye, Jiamin;Dai, Aijun;Wang, Jidong;Zhang, Hui;Zhang, Yongchun;Wang, Jing;Zhang, Licheng;Li, Juan;Zhang, Mingqing;Ye, Jiamin;Wang, Jidong;Zhang, Yongchun;Ye, Jiamin;Dai, Aijun;Chen, Danyan
关键词：Ipomoea batatasL; Long-term organic fertilization; Plant yield; Potassium uptake and utilization; Soil aggregates-associated potassium
