文献类型： 外文期刊

作者： Xu Zeyu ¹ ; Dang Anlei ¹ ; Deng Bihua ¹ ; Zuo Xiaoxin ¹ ; Lu Yu ¹ ; Yang Ping ³ ; Yin Wenzhu ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China

2.Guotai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Jiangsu, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

关键词： graphene oxide; quantum dot; adjuvant; adsorption; antibody

期刊名称：CHINESE JOURNAL OF INORGANIC CHEMISTRY （ 影响因子：0.7； 五年影响因子：0.5 ）

ISSN： 1001-4861

年卷期： 2024 年 40 卷 6 期

页码：

收录情况： SCI

摘要： The improved Hummers method was used to obtain GOQDs from flake graphite, which contained rich hydroxyl and carboxyl groups. With the as-fabricated GOQDs in hand, we constructed a GOQDs/OVA nano-vaccine using chicken ovalbumin (OVA) as a model antigen to evaluate the immune efficacy and safety. Results showed that GOQDs/OVA nano-vaccine had high water dispersibility and stability with a diameter of around 5 nm for 30 d. The maximum loading capacity of GOQDs for OVA was about 500 mg center dot g(-1), and release rates of OVA were 74.65% and 56.93% in pH 5.5 and 7.4 after 24 h, respectively, displaying pH stimulus responsive release merits. With the concentration of GOQDs below 500 mu g center dot mL(-1), the biosecurity of GOQDs indicated that they were not causing hemolysis, cell damage, and pathological changes in important tissues. After immunization, GOQDs/OVA nano-vaccines could excite the high levels of immunoglobulin G (IgG), immunoglobulin G1 (IgG1), and immunoglobulin G2a (IgG2a) antibodies and improve secretions of interleukin-1 beta (IL-1 beta), interleukin-4 (IL-2), interleukin- 4 (IL-4), interleukin-6 (IL- 6), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma), compared with the group of OVA alone. Meanwhile, GOQDs promoted an increase in the percentage of CD4+ and CD8+ T lymphocytes in the spleen.